摘要
目的 摸索高效液相色谱法（HPLC）测定宣肺颗粒中岩白菜素含量的方法。方法：采用C18柱，以甲醇-水-冰醋酸（18：81：1）（V／V）为流动相；检测波长275nm；样品经超声波震荡50min提取滤过。结果 线性范围0．43～2．15μg／ml，RSD=0．41％；加样回收率98．33％，RSD=2．68％；阴性对照无干扰。结论 本法操作简便、快速，结果准确可靠，可作为宣肺颗粒中岩白菜素的含量测定方法。
Objective.. To establish a content determination method of bergenin in Xuanfei granule by HPLC. Methods： Used a column packed with C18 and a mixture of methanol-water-ice acetic acid（ 18： 81： 1）as the mobile phase, detection wavelength was 275nm. Samples were abstracted in ultrasonic wave for 50 minutes and then filtered. Results：The linear range was 0. 43～2. 15（RSD= 0. 41%）,The mean recovery was 98. 33% （RSD= 2. 68%）, and the blank sample had no interference. Conclusion： The method is simple,quick and accurate. It can be used as a quality content method of Xuanfei granule.
出处
《湖北中医学院学报》
2006年第3期18-20,共3页
Journal of Hubei College of Traditional Chinese Medicine
