摘要
将番茄果实特异性启动子通过PCR的方法扩增出来,然后将其替代了pBIN438的CaMV35S启动子,并将乙肝表面抗原的S基因连接到载体上,以获得番茄果实特异性表达乙肝表面抗原的高效植物表达载体。
In this research we amplified the fruit - specific promoter of tomato by PCR, then substituted it for the CaMV35S promoter of pBIN438, and linked the gene of S of Hepatitis B surface antigen to this vector , in order to acquire Plant Expression Vector in which the tomato fruit - specific express Hepatitis B surface antigen.
出处
《长春理工大学学报（自然科学版）》
2006年第3期72-75,共4页
Journal of Changchun University of Science and Technology(Natural Science Edition)
基金
吉林省科技厅基金项目(200405-5)
关键词
番茄
果实特异性启动子
乙肝表面抗原
植物表达载体
tomato
fruit- specific promoter
Hepatitis B surface antigen
plant expression vector
