摘要
本实验利用结扎沙土鼠双侧颈总动脉制成的急性脑缺血模型,观察了缺血60min,重灌流30min后脑组织含水量、脂质过氧化物含量以及Na^+,K^+-ATP酶、SOD的活力变化.实验中发现,缺血60min以内,组织含水量增加,Na^+,K^+-ATP酶和SOD的活力降低。在继以30min的重灌流之后,Na^+,K^+-ATP酶与SOD的活力继续下降,而脂质过氧化物含量急剧升高。实验结果提示缺血重灌流以后产生自由基对缺血后的脑组织产生损害作用。提前给动物使用别嘌呤醇,能够对缺血后Na^+,K^+-ATP酶、SOD起到部分保护作用。
The effect of bilateral carotid occlusion for 60 min and reperfusion for 30 min on levels of water and lipid peroxides (LPO) and activities of Na+, K+ -ATPase and superoxide dismutase (SOD) in the forebrain was studied in gerbils (Meriones unguiculatus). Luring occlusion, increased water content and decreased activities of Na+, K+-ATPase and SOD were observed. After reperfusion, the activities of Na+, K+ -ATPase and SOD continued decreasing, but LPO elevated apparently. These results support a hypothesis that the free radicals may contribute to the damage of ischemic brain tissue. Pretreatment of gerbils with allopurinol offered protection to cerebral Na+, K+ -ATPase and SOD subsequent to secondary ischemia.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第1期49-52,共4页
Academic Journal of Second Military Medical University
关键词
脑缺血
自由基
损害作用
cerebral ischemia
Na^+, K^+-ATPase
superoxide-dismutase
lipid peroxides
allopurinol
