摘要
采用析因设计及ＬＵＺＥＸ－Ｆ型彩色图像分析仪判定内皮细胞融合度等手段，研究明胶，纤维连接蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ），人血浆和２０％人体白蛋白，单一或混合物，共３１种做为预衬物，对促进人脐带静脉内皮细胞贴附Ｄａｃｒｏｎ人工血管生长的作用。结果：１％明胶＋血浆＋３００μｇ／ｍｌ纤维连接蛋白、１％明胶＋血浆＋７５０μｇ／ｍｌ纤维连接蛋白、１．５％明胶＋７５０μｇ／ｍｌ纤维连接蛋白三种预衬物对促进人脐带静脉内皮细胞贴附Ｄａｃｒｏｎ人工血管生长的功效最好。同时反映出：随明胶浓度的升高，促进内皮细胞贴壁生长的能力增强；明胶与血浆、明胶与ＦＮ、明胶与２０％人体白蛋白及血浆与２０％人体白蛋白间有的较强的协同促进内皮细胞贴壁生长的作用；ＦＮ与２０％人体白蛋白混合不但没有协同促进内皮细胞贴壁生长的作用，反而会影响其它因素间协同促进作用；２０％人体白蛋白单独做为预衬物没有促进内皮细胞贴壁生长的作用。
Division factor analysis design and LUZEX F type color image analysis computer in measuring the confluence of endothelial cells(EC) were used to investigate the effect of thirty one kinds of precoating substances, which come from monoconcentrations or combinations of gelatin (0.5%, 1%, 1.5%), fibronectin (300 μg/ml,750 μg/ml), human plasm and 20% human albumin, on improving the attachment and proliferation of human umbilical vein endothelial cells (HUVEC) on Dacron artificial vascular surface. The results indicated that 1% gelatin+human plasma +300 μg/ml fibronectin, 1% gelatin+ human plasma+750 μg/ml fibronectin, were the best of all the precoating substances, which have better effect on improving the attachment and porliferation of HUVEC. The results also showed that the gelatin's effect on the attachment and proliferation of HUVEC got better when its concentration increased. Gelatin and plasma, gelatin and fibronectin, gelatin and 20% human albumin, plasma and 20% human albumin had strong synergistic effect on improving EC attachment and proliferation whereas fibronectin and 20% human albumin not only had not the synergism on improving the attachment and proliferation of EC, but decrease the effect of other procoating substances. Twenty percent of human albumin alone had no effect on improving the attachment and proliferation of EC.
出处
《中国医科大学学报》
CAS
CSCD
1996年第6期589-592,共4页
Journal of China Medical University
关键词
人工血管
内皮细胞
细胞培养
预衬物
Dacron vascular graft
endothelium
cell culture
precoating substance
