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产丁二酮的嗜热链球菌的筛选和鉴定 被引量：2

Screening and Identification of S. thermophilus Producing Diacetyl
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摘要 从酸马奶中分离出五株链球菌,其中KM18的丁二酮的产量为20mg/mL。利用PCR扩增该菌的16SrRNA基因,将测序结果同该属内菌株的16SrRNA基因序列作多序列比较,并建立链球菌属的系统发育树。结果表明,KM18的16SrRNA基因序列同S.thermophilus的同源性百分比为98.2%。综合系统发育树的结果,将KM18鉴定为S.thermophilus。菌株KM18的16SrRNA基因序列已经在GENBANK申请国际序列注册号,为DQ176426。 To identify the strain of KM18 producing diacetyl, the 16S rRNA gene was amplified and sequenced. Then a phylogenetic tree was constructed by multiple alignments of 16S rRNA gene Sequences of S.treptococcus. The results indicated that KM18 was classified as S. therrnophilus.
作者 刘芳 王玉堂 霍贵成
机构地区 东北农业大学乳品科学教育部重点实验室
出处 《乳业科学与技术》 2006年第6期272-275,共4页 JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金 国家"863计划"课题资助(2002AA248041)。
关键词 丁二酮 16S RRNA基因 聚类分析 diacetyl 16S rRNA gene cluster analysis
分类号 TS201 [轻工技术与工程—食品科学]
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参考文献8

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乳业科学与技术
2006年 第6期

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