摘要
应用PEF成功从奶牛脾脏中提取非特异性转移因子,并与常规提取法和复合酶提取法进行了比较。经理化特性分析,奶牛脾脏转移因子是以低分子多肽(<10kD)为主的混合物,含有少量核酸和氨基酸。实验表明,将物料溶解于6倍质量的磷酸盐(pH7.0)缓冲液中,在脉冲电场强度为25kV/cm,脉冲数为16μs,和流速为2mL/min的条件下,提取液中低分子多肽的含量有最大值9.85mg/mL。PEF提取率是常规方法的2倍,复合酶法的1.47倍。PEF法耗时短,操作简单,成本低。因此,PEF提取是一种很有希望的提取非特异性奶牛脾脏转移因子方法。
A high throughput lion-single transter factor （TF） extraction method was developed from Milch cow spleen with pulsed electric fields （PEFi and the non-single TF produced were composed of most Low molecular polypeptide and a little nucleic acid and amino acid. The largest polypeptide extraction was 9.85mg/ml when materials suspended in phosphate buffer （pH7.0） was treated for 16μs at 25 kV/cm, flow velocity 2mL/min, and diluted six times in bipolar mode. Finally, PEF processing was 2 times more effective than untreated extraction and 1.47 times than compound enzyme extraction. This method can effectively decrease the time, cost and handling error of the non-single TF extraction stage. So PEF method is a novel and promising method to extract non-single TF.
出处
《乳业科学与技术》
2006年第6期290-292,共3页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
吉林省科技发展计划项目20050202-3。
关键词
高压脉冲电场
奶牛脾脏
非特异性转移因子
低分子多肽
Pulsed electric field
milch cow spleen
non-single transfer factor
Low molecular polypeptide
