摘要
目的研究7日龄新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)后海马神经组织生长相关蛋白(growth-associated protein,GAP-43)及其mRNA的表达变化。方法按Rice法制备SD大鼠HIBD模型,并以正常新生大鼠为对照。以免疫组织化学和RT-PCR法检测不同时相点海马组织GAP-43及其mRNA的表达。结果新生大鼠HIBD后海马GAP-43及其mRNA表达较同日龄正常大鼠明显增高,表达高峰分别在损伤后第2周和第3周。结论新生大鼠HIBD后海马GAP-43及其mRNA表达增高,可能与海马损伤后修复有关。
Objective To observe the expression of growth-associated protein-43 （GAP-43） in the hippocampus of neonatal rats with hypoxic-ischemic brain damage （HIBD）. Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD （n =8 at each time point for each group）. Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第21期2160-2162,共3页
Journal of Third Military Medical University
关键词
脑缺血
脑缺氧
生长相关蛋白
海马
大鼠
cerebral hypoxia
cerebral ischemia
growth-associated protein
hippocampus
rat
