摘要
生物组织工程化气管是利用组织工程学的原理和技术在体外构建出具有气管主要生理功能的一种具有生物活性的人工气管,作为气管缺损外科修复的气管替代物。它的构建过程是,首先选取一定数量的经体外培养扩增种子细胞,把它们种植在生物相容性好、可降解的支架材料上;然后将此细胞材料复合物置于生物反应器当中或自体皮下,经过一段时间的诱导分化和培养即形成组织工程化气管。与其它种类的气管替代物相比,组织工程化气管具有无免疫原性、无排斥反应、有生物活性等优点,这将为寻找一种适合机体内环境的气管移植材料带来一条有希望的途径。但是该领域的研究工作还面临着诸如组织工程化气管的血管化、体外培养和植入后的感染等难题,要将组织工程化气管真正应用到临床,还有很长的路要走。我们从细胞支架的研究、种子细胞的选择和组织工程化气管的构建这三方面对组织工程化气管的研究进行综述。
Tissue engineering trachea is an artificial trachea with biological activity, which is constructed in vitro by using tissue engineered principle and technology, and is a tracheal prosthesis for replacing large circumferential defect of the trachea. The course of its construction is as follows. First, seeding cells are cultured and expanded in vitro. Then they are collected, counted and seeded onto the biomaterial scaffold of tissue consistent and biodegradation. Finally, the biomaterial-eells construction is implanted into bio-reaetion device or one＇s subcutaneous layer. The tissue engineering trachea could he constructed after cultured certain times. Compared with other artificial trachea, the tissue engineering trachea has more advantages, such as nonimmunogenieity, no side-effects related to foreign graft materials, and biologic activity. This will bring some hope to look for an appropriate graft material. However, the study about it is still faced with some difficult problems, such as vaseularized trachea, culturing in vitro, and prevention of infection in trachea prosthesia. So there will he long time for tissue engineering trachea to apply clinical tracheal transplantation successfully. This assay has reviewed the study about tissue engineering trachea from three sides——the source of seeding cells, the research about biomaterial scaffold, and the construction of tissue engineering trachea.
出处
《中国胸心血管外科临床杂志》
CAS
2006年第5期338-343,共6页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
组织工程
气管
移植
Tissue engineering
Trachea
Transplantation
