摘要
目的：从三七茎叶中提取总黄酮。方法：采用正交试验法研究三七茎叶总黄酮的提取工艺,考察了浸提用水的pH值、浸提温度、浸提时间、浸提次数、液料比五个因素对三七茎叶总黄酮提取率的影响。结果：确立了三七茎叶总黄酮最佳提取条件为：用pH10的稀碱水作溶剂,液料比30：1,浸提温度为80℃,回流提取2次,提取时间为每次40min。
Objective： In order to extract the total flavonoids from the the stalk and leaves of Panax notoginseng, extraction technology of total flavonoids was studied by orthogonal experiment.Methods：Effects of alkali - water pH, extraction temperature, extraction times, extraction times and ratio of liquid to material on flavonoids eantents were investigated. Results：Optimum extraction conditions were determined as follows： alkali - water（pH = 10） as the solvent, ratio of liquid to material 30：1, two times of reflux extraction at 80℃ ,each for 40 minutes.
出处
《生物技术》
CAS
CSCD
2006年第5期65-67,共3页
Biotechnology
基金
云南省教育厅科学研究基金项目资助(520505C)
关键词
三七茎叶
总黄酮
提取
正交试验法
the stalk and leaves of Panax notoginseng
total flavonoids
extraction
orthogonal experiment
