摘要
目的：为了提高脂肪酶的产量及更好地应用脂肪酶。方法：采用单因子实验与均匀设计相结合的方法,对青霉Penicil- lium sp．TS414发酵生产脂肪酶的条件进行了优化。结果：在实验优化后的最适产酶培养基中,碳源为1．4%蔗糖,氮源为7．0%豆饼粉,起始pH8．0。均匀设计优化后的产酶水平(315．1U/mL)比优化前(101．5U/mL)提高了约2倍。Penicillium sp．TS414脂肪酶能够有效地催化大豆油转酯化合成脂肪酸甲酯(生物柴油),反应72h后,脂肪酸甲酯的最终得率在96%左右。结论：Penicillium sp．TS414产生的脂肪酶在生物柴油的工业化生产方面,具有潜在的应用前景。
Objective： It was to promote lipase production and use the enzyme more effectively. Methods： single- factor experiment was combined with the uniform design to optimize the fermentation conditions of a lipase from Penicillium sp ,TS414. Results： the optimal medium for lipase production was： 1.4% sucrose as carbon source, 7.0% soybean meal as nitrogen source, initial pH8.0. Under the optimal fermentation conditions, Penicillium sp .TS414 produced about two times more lipase（315.1U/mL） than pre optimization （101.5U/mL）. Additionally, Penicillium sp. TS414 lipase could be effectively used for transesterification of the soybean oil into the methyl esters（biodiesel）. The overall yield of the methyl esters was approximately 96% after 72h ours. Conclusion： Penicillium sp. TS414 lipase had a potential use in the industrial production of biodiesel .
出处
《生物技术》
CAS
CSCD
2006年第5期67-70,共4页
Biotechnology
