摘要
瞄准：在成年老鼠的睾丸由 FA 对氧化损坏在睾丸和维生素Ｅ(VE ) 的保护的效果上调查甲醛(FA ) 的效果。方法：三十只老鼠随机被划分成三个组：(1 ) 控制；(2 ) FA 处理组织(脂肪) ；并且(3 ) 胖 + VE 组织。胖、胖的 + VE 组被吸入在 10 mg/m (3 ) 的集中暴露于 FA 2 个星期。另外，胖 + VE 组在 2 星期的 FA 处理期间是口头上地管理的 VE。在精子的处理，在睾丸的组织病理学说、生物化学的变化，以及数量和质量以后，被观察。结果：精子的阴囊的重量，数量和质量，超级氧化物歧化酶(草皮)的活动，而 malondialdehyde ( MDA )的水平显著地在控制组与那些相比在胖组在老鼠的睾丸被增加，谷胱甘肽过氧化物酶( GSH-Px )和谷胱甘肽( GSH )显著地被减少。VE 处理在胖 + VE 组恢复了这些参数。另外，有 hematoxylin 曙红的显微镜学(他) 染色证明细精管萎缩的、生精的上皮细胞瓦解了，在在胖组和 VE 处理的老鼠的 shed 显著地在胖 + VE 改进了阴囊的结构组。结论：在由导致氧化应力的成年老鼠的阴囊的结构和功能，和这损坏能部分是的 FA 破坏由 VE 逆行。
Aim： To investigate the effect of formaldehyde （FA） on testes and the protective effect of vitamin E （VE） against oxidative damage by FA in the testes of adult rats. Methods： Thirty rats were randomly divided into three groups： （1） control; （2） FA treatment group （FAt）; and （3） FAt ＋ VE group. FAt and FAt ＋ VE groups were exposed to FA by inhalation at a concentration of 10 mg/m^3 for 2 weeks. In addition, FAt ＋ VE group were orally administered VE during the 2-week FA treatment. After the treatment, the histopathological and biochemical changes in testes, as well as the quantity and quality of sperm, were observed. Results： The testicular weight, the quantity and quality of sperm, the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and glutathione （GSH） were significantly decreased whereas the level of malondialdehyde （MDA） was significantly increased in testes of rats in FAt group compared with those in the control group. VE treatment restored these parameters in FAt ＋ VE group. In addition, microscopy with hematoxylin-eosin （HE） staining showed that seminiferous tubules atrophied, seminiferous epithelial cells disintegrated and shed in rats in FAt group and VE treatment significantly improved the testicular structure in FAt ＋ VE group. Conclusion： FA destroys the testicular structure and function in adult rats by inducing oxidative stress, and this damage could be partially reversed by VE.
基金
grants from the SciTechnical Development Project of Shaanxi Province, China （2005K15-G2）
Traditional Chinese Project of Shaanxi Province, China （2005024）
Natural Science Foundation of Xi＇ an Jiaotong University （573026）.
关键词
维生素E
保护作用
氧化损伤
甲醛
小鼠
formaldehyde
vitamin E
testis
rats
oxidative stress
reproductive toxicity
