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青蒿琥酯抗鸭乙型肝炎病毒的体内实验 被引量:6

Study of the antiviral effect on duck hepatitis B virus of artesunate
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摘要 目的观察青蒿琥酯体内抗鸭乙型肝炎病毒(duck hepatitis B virus,DHBV)的作用。方法将DHBV DNA阳性麻鸭随机分为青蒿琥酯大、中、小剂量组、拉米夫定组和空白对照组,分别给予相应药物进行干预。用药前、用药第71、4、21、28天,及停药第7天,取静脉血用实时荧光定量PCR法检测血清DHBVDNA含量。治疗前后取肝脏组织作病理学检查。结果拉米夫定组于用药后,血清DHBV DNA水平迅速降低,停药后立即反跳。青蒿琥酯大剂量组第21、28天DHBV DNA抑制率与拉米夫定组相似,停药后仍有一定的抑制作用。中、小剂量组与空白对照组比较有一定的病毒抑制作用。病理改变各组在统计学上无明显差异。结论大剂量青蒿琥酯对DHBV DNA的抑制率与拉米夫定相似,维持时间长,安全,但其抗HBV作用尚需进一步研究。 Objective To observe the inhibiting effect of artesunate on duck hepatitis B virus in vivo, Methods Ducks with congenitally infection of DHBV were randomly divided into five groups: high, medittm, low-dose artesunate treated group, lamivudine-treated group and untreated group, The ducks in the lamivudine-treated group were fed lamivudine with a dose of 50mg/kg once, Ducks in the three-dose artestmate-treated groups were treated with different doses of decoction of this medicine for 28 days respectively, The serum content of DHBV DNA was determined by FQ-PCR method before and 7 days after the treatment, and on the 7th, 14th, 21st and 28th day of the treatment, The histopathological changes of liver were evaluated before "and after the treatment. Results Lamivudine showed a rapid inhibiting effect on DHBV DNA, but this effect didn't last long, and the serum level of DHBV DNA increased again after treatment. The serum level of DHBV DNA dropped markedly in the high-dose artesunate-treated group on the 21st and 28th day. Lowdose andd medium-dose artesanate also showed certain inhibiting effect on DHBV DNA. The features of liver histopathological changes in the artesunate treated groups showed no statistical significance as compared with that in the untreated group. Conclusions Artesunate showed interesting anti-DHBV activity in vivo. The inhibition in DHBV DNA of artesunate was dose-dependent, continuous and safe. Moreover, artesunate had anti-HBV effects, which warrants further evaluation of artesunate as antiviral agents against HBV infection.
出处 《国际流行病学传染病学杂志》 CAS 2006年第5期308-310,323,共4页 International Journal of Epidemiology and Infectious Disease
关键词 鸭乙型肝炎病毒 青蒿琥酯 中药 实时荧光定量PCR 动物实验 Duck hepatitis B virus Artesunate Traditional Chinese medicine Fluorescent quantitative real-time PCR Animal experimentation
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