摘要
目的建立高效液相色谱法测定环孢霉素眼用原位凝胶的含量。方法色谱柱为Thermoelectory C18，流动相：乙腈-水（90：10），检测波长210m，流速0．7mL·min^-1。样品用甲醇溶解并用0．45μm微孔滤膜过滤。结果在5～60μg·mL^-1范围内，环孢素对照品的峰面积（Y）与其浓度（X）呈良好的线性关系，r=0．9996，高、中、低3种浓度平均回收率99．3％、99.8％、98．9％（n=3），日内、日间RSD分别为1．45％，1．93％（n=5）。结论该法快速、灵敏、准确、专属性高。
OBJECTIVE To determine the contents of cyclosporine in ophthalmic situ-forming gel with HPLC. METHODS A reversed phase high performance liquid chromatographic method was developed. Cyclosporine was chromatographed on thermoelectory C18 column and detected at 210 nm. The mobile phase was acetonitrile., water （90 ： 10）, the flow rate was 0.7 mL · min^-1. Samples were dissolved with methanol, and then was filtered with 0.45μm filter membrane. RESULTS The linearity of cyclosporine was 5～60μg·mL^-1 and the coefficient of correlation was 0. 999 6. The average recoveries of cyclosporine were 99.3%, 99.8%, and 98.9%（n=3）. The within day and between day RSD were 1.45% and 1.93% （n=5） respectively. CONCLUSIONS The method is rapid, sensitive and efficient for the determination of CsA in ophthalmic situ-forming gel.
出处
《中南药学》
CAS
2006年第5期354-355,共2页
Central South Pharmacy
