摘要
目的:应用近红外漫反射光谱技术和数据分析软件,对酒炖熟地黄饮片粉末中还原糖的含量进行快速测定。方法:以还原糖测定仪测定酒炖熟地黄饮片粉末样品中还原糖的含量,采集其近红外光谱数据。应用偏最小二乘法(PLS)对建立还原糖含量测定的校正模型。结果:内部交叉验证和外部检验集样品对模型验证结果表明,真实值与预测值之间的相关系数(r2)分别为89.02和88.47,内外验证均方差RMSECV分别为0.962和0.987。配对比较t检验,真实值与预测值之间均无显著性差异。结论:本研究所建立的模型可以很好的预测酒炖熟地黄饮片中指标成分还原糖的含量。该方法简便可行,尤其适用于生产中间控制和大批量产品的检测。还可用于生产过程的质量控制,对于其它的中药材、中成药制剂等的指标成分测定也有一定的参考价值。
Objective：To determine rapidly the reducing sugar content in the powder of decoction pieces of Shu Dihuang stewing with mine bv FT-NIRS and data analysis software. Methods：The powders of samples were analyzed by reducing sugar radiometer and FT-NIR spectra. The prediction model was set up based on PLS（ partial least square）and was used to predict reducing the sugar content in the unknown samples. Results：Cross validation and test samples determination showed that the correlation coefficient of this prediction model were 89.02 and 88.47, the RMSECV were 0. 962 and 0. 987 respectively. And t-test showed that there was no significant difference between the true data and predictive value. Conclusion：The prediction model could be used to predict reducing sugar content in the powder of decoction pieces of Shu Dihuang stewing with mine. This method is feasible to determine reducing sugar content in Shu Dihuang with convenient operation. It can offer reference to contents determination of other Chinese crude drugs and Chinese patent medicine.
出处
《中药材》
CAS
CSCD
北大核心
2006年第10期1035-1038,共4页
Journal of Chinese Medicinal Materials
基金
河南省自然科学基金资助项目(004025000)
关键词
近红外漫反射光谱
偏最小二乘法
熟地黄
还原糖
含量测定
Near-infrared diffuse reflectance spectroscopy
PLS
Shu Dihuang
Reducing sugar
Content determination
