摘要
以杉木成熟合子胚为起始外植体,研究基本培养基、不同种类的细胞分裂素及生长素对杉木器官发生和体胚发生及不定芽生根的影响,并用石蜡切片法观察不定芽和体胚的发生和发育过程.结果表明：DCR＋6-BA 1.0mg·L-1＋TDZ 0.002 mg·L-1＋NAA 0.1 mg·L-1对成熟合子胚诱导不定芽最有效;DCR＋6-BA 1.0 mg·L-1＋TDZ 0.003mg·L-1＋NAA 0.1 mg·L-1是成熟合子胚诱导体胚的最佳培养基;不定芽在DCR基本培养基中可有效伸长;1/4MS＋IBA 0.2 mg·L-1＋NAA 0.1 mg·L-1对不定芽生根最有效.石蜡切片观察表明：不定芽有2种发生方式,一种是表皮及表皮下的3～4层细胞分生组织化发育而成,另一种是外植体内部的小维管束脱分化发育而成.2种发生方式均为直接器官发生途径.
Using mature zygotic embryos as initial explants, the effect factors including basal medium and plant hormone on organogenesls, somatic embryogenesis and adventitious buds induction roots were investigated respectively. The origin and development of somatic embryos and adventitious buds were observed through the paraffin method. The results indicated that the medium of DCR＋6-BA 1.0 mg· L^-1 ＋ TDZ 0.002 mg· L^-1 ＋ NAA 0.1 mg· L^-1 was optimal for mature zygotic embryos to induce adventitious buds. The optimal medium for mature zygotic embryos to induce somatic embryos was DCR ＋ 6-BA 1.0 mg· L^-1 ＋ TDZ 0.003 mg·L^-1 ＋ NAA 0.1 mg· L^-1. Adventitious buds can elongate effectively on the DCR medium without plant hormone. 1/4MS ＋ IBA 0.2 mg·L^-1 ＋ NAA 0.1 mg·L^-1 was the optimal medium for adventitious buds to root. Histological observation indicated that there were two ways in which adventitious buds originate. The first way of origination was from meristematic tissue developed from epiderm cells and three or four liners under epiderm. The other was that the little vascular bundle dedifferentiated into meristematic tissue, which in turn developed into adventitious buds. Both of the two ways were direct organogenesis.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2006年第9期29-33,I0002,共6页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(30170745)
江苏省高校省级重点实验室开放课题(KJS03035)资助
关键词
杉木
成熟合子胚
器官发生
体胚发生
Cunninghamia lanceolata
mature zygotic embryos
organogenesis
somatic embryogenesis
