摘要
利用综3、87-1、郑58、昌7-2、137和K12等6个骨干自交系玉米幼胚进行愈伤组织诱导并建立再生体系。结果显示,2,4-D对玉米幼胚愈伤组织的诱导形成是必需的,浓度在2.0～2.5 mg/L;6-BA不利于愈伤组织的形成,但能促进幼胚直接长芽、长根,尤其以郑58最明显。材料综3、郑58、K12诱导愈伤组织比较容易,诱导率均≥85%;材料137和87-1表现中等,诱导率约在50%～60%;材料昌7-2表现最差,愈伤组织诱导率≤25%。
Callus was induced from immature embryos of six maize inbred lines on N6 medium supplemented with various phytohormones. The results indicated that 2,4-dichlorophenoxyacetic acid （2,4-D） was necessary in inducing callus, and its concentration 2.0 - 2.5 mg/L. Different maize varieties had different regeneration capacities, the induction rates of Zong 3, Zheng 58 and K12 were over 85%; that of 137 and 87-1 ranged from50% to 60%; but that of the chang 7-2 was low 25%.
出处
《玉米科学》
CAS
CSCD
北大核心
2006年第5期71-73,86,共4页
Journal of Maize Sciences
关键词
玉米
幼胚
愈伤组织
诱导
再生体系
Maize
Immature embryos
Callus
Initiation
Regeneration system
