摘要
目的探讨乙型肝炎e抗原(HBeAg)、前S1抗原(Pre-S1)、前S2抗原(Pre-S2)与其病毒DNA(HBVDNA)的关系。方法收集268例乙型肝炎患者血标本,以荧光定量PCR法检测HBV DNA,时间分辨荧光免疫分析法检测HBeAg,酶联免疫吸附法检测Pre-S1、Pre-S2。结果HBV DNA阳性组HBeAg、Pre-S1、Pre-S2阳性率分别为48．2％、76．4％、100％,HBV DNA阴性组分别为1．6％、36．3％、32．3％,两组间每指标阳性率比较,差异均有显著性(均P<0．01)。结论HBeAg、Pre-S1、Pre-S2与HBV DNA关系密切,以HBV DNA阳性为参照标准,反映病毒复制的敏感性Pre-S2最高,其次为Pre-S1,HBeAg较低;Pre-S1、Pre-S2可作为HBV DNA的补充指标。
Objective To study the relationship between the hepatitis B e-antigen （HBeAg）, Pre- S1 antigen （Pre-S1）, Pre-S2 antigen （Pre-S2） and DNA of hepatitis B virus （HBV）. Methods The blood samples of 268 cases of viral B hepatitis were collected. The HBV DNA of all samples were tested by fluorescent-quantitating PCR method, and HBeAg were assayed by time-resolved fluoroim-munoassay method, and their Pre-S1 and Pre-S2 were assayed by enzyme linked immunosorbentassay method. Results The positive rates of HBeAg, Pre-S1 and Pre-S2 in HBV DNA positive group were 48.2%, 76.4% and 100% respectively, and 1.6%, 36.3% and 32.3% respectively in HBV DNA negative group. There was significantly difference between the HBeAg, Pre-S1 and Pre-S2 positive rates of the two groups （Chi-square test, P 〈0.01）. Conclusions There was positive relationship between the HBeAg, Pre-S1, Pre-S2 and DNA which all were indicators of HBV reproduction. Comparing to HBV DNA , Pre-S2 was the most, Pre-S1 the second, and HBeAg the third sensitive indicator for evaluating HBV reproduction. Pre-S1 and Pre-S2 could be used as the supplementary indicator for the reproduction of HBV.
出处
《国际放射医学核医学杂志》
2006年第4期232-234,共3页
International Journal of Radiation Medicine and Nuclear Medicine
