摘要
目的:探讨尿激酶型纤溶酶原激活剂(u-PA)、其受体(u-PAR)和其抑制剂-1(PAI-1)在PCOS发病机制中的作用。方法:采用睾酮或孕酮联合hCG刺激建立PCOS大鼠模型;免疫组化S-P法测定u-PA、u-PAR和PAI-1在模型动物卵巢组织中的分布与表达。结果:u-PA、u-PAR在PCOS组颗粒细胞和泡膜细胞中的染色比正常对照组明显减弱(P<0.05),PAI-1在泡膜细胞及间质细胞中的染色均比正常对照组明显增强(P<0.05)。结论:u-PA、u-PAR及PAI-1的表达异常可能参与PCOS的发病。
Objective： To explore the role of u-PA, u-PAR and PAI-1 in the pathogenesis. Methods： Testosterone synchronized rats were used as the model of PCOS by injecting human chorionic gonadotropin; u-PA, u-PAR and PAI-1 were determined by immunohistochemical staining in the rat＇s ovaries. Results： Immunostaining of u-PA and u-PAR in the granulosa theca cells of PCOS were obviously weaker than that of normal control （P〈0.05）. Immunostaining of PAI-1 in the theca interstitial theca cells of PCOS was obviously stronger than that of normal control （P〈0.05）. Conclusion： The disruption of u-PA system may be involved in the pathogenesis of PCOS.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2006年第10期579-583,共5页
Reproduction and Contraception
基金
哈尔滨医科大学研究生创新基金项目
