摘要
目的探讨钝顶螺旋藻多糖(polysaccharide fromSpirulina platensis,PSP)抗单纯疱疹病毒及乙型肝炎病毒的作用及可能机制,为进一步开发该药提供理论依据。方法以不同剂量的PSP分别作用于HSV-1及HSV-2病毒复制周期的各个环节,以病毒半数感染量(TCID50),细胞病变效应(CPE),蚀斑形成单位(PFU),MTT法作为评价指标,判断PSP的抗病毒效果;ELISA法定量检测PSP对HepG2 2.2.15细胞HbsAg,HBeAg分泌的影响;FQ-PCR检测PSP对HBV-DNA合成的影响。结果PSP对Vero细胞及HepG2 2.2.15细胞毒性极低,对HSV-1及HSV-2均无直接灭活作用,可阻滞HSV-1及HSV-2病毒的吸附并抑制感染细胞内病毒的复制,但不影响病毒的释放。在HepG2 2.2.15细胞培养中PSP可显著抑制HB-sAg、HBeAg的分泌以及HBV-DNA的合成,并具有量效和时效关系。结论PSP抗HSV-1及HSV-2病毒作用的机制与抑制病毒吸附和感染细胞内病毒的生物合成有关,而PSP抗HBV作用的机制则与抑制HBV抗原分泌及病毒DNA复制有关。
Objective To explore the effects and mechanisms of PSP against HSV and HBV and to provide a theoretical support for developing new drugs. Methods PSP in various concentrations was applied to different phases of HSV-1 and HSV-2 replication cycles respectively, finally the virus infectivity （TCID50）, cytopathic effect （CPE）, MTT staining method for viable cells（MTT assay） were used as indexes to evaluate the effects of PSP on HSV-1 and HSV-2. The inhibitive effect of PSP on secretion of HBeAg and HBsAg were tested by ELISA;The quantity of HBV-DNA was detected by real-time fluorescence quantitative PCR（FQ-PCR）. Results PSP had little cytotoxic effect on Vero cells （TC50=1750 mg·L^-1）, and could not directly inactivate HSV-1 and HSV-2, but inhibit HSV-1 and HSV-2 absorption and replication in host cells. The polysaccharides did not show inhibitive effect on HSV release. PSP also had little cytotoxic effect on HepG2 2. 2. 15 cells （TG50〉3200 mg·L^-1）, and could suppress HBeAg and HBsAg secretion and lower the HBV- DNA level cultured in HepG2 2.2.15 cell line in a dose and time-dependent manner. Conclusion Some of the mechanisms of PSP against HSV-1 and HSV-2 may be explained by the considerable inhibition of virus absorption and virus replication in cells. Some of the mechanisms of PSP against HBV may be explained by inhibition of the secretion of HBV-antigen and the replication of HBV- DNA.
出处
《中国海洋药物》
CAS
CSCD
2006年第5期19-24,共6页
Chinese Journal of Marine Drugs
基金
青岛市科技计划项目(No.02-2-kj-yi-31)
关键词
螺旋藻多糖
钝顶螺旋藻
单纯疱疹病毒
乙型肝炎病毒
抗病毒作用
polysaccharides from Spirulina platensis （PSP）
Spirulina platensis
herpes simplex virus （HSV）
hepatitis B virus （HBV）
antiviral activities
