摘要
目的建立高氧致中度肺损伤大鼠动物模型,研究其肺泡液体清除率(AFC)的变化。方法高氧大鼠暴露于90%的氧浓度,分别于24和48h测定AFC、总肺水量和肺血管外肺水量。并检测氨氯吡咪、哇巴因及特布他林对高氧大鼠及正常对照组大鼠AFC的影响。结果与对照组大鼠比较,高氧大鼠总肺水量和肺血管外水量明显增加(P<0.01),而AFC没有明显变化(P>0.05)。氨氯吡咪、哇巴因明显降低AFC(P<0.05),特布他林对高氧大鼠AFC的影响与对照组大鼠比较差异无显著性(P>0.05)。结论轻、中度肺损伤时AFC没有明显变化。
[Objective] To establish the animal model of moderate lung injury caused by hyperoxia in rats, and measure the alveolar fluid clearance. [Methods] Rats were exposed to 90% oxygen. AFC, TLW, and EVLW were calculated in rats exposed to hyperoxia for 24 h and 48 h. The effect of amiloride. ouabain and terbutalin on AFC was tested in hyperoxic exposure and room air-exposed rats. [Results] There was significant difference in TLW and EVLW in rats with hyperoxic exposure compared to room air exposure. However, AFC did not change. Amiloride and ouabain can reduce the AFC in both room air-exposed and hyperoxic-exposed rats. There was no significant difference in the magnitude of the terbutalin-stimilated AFC in rats with hyperoxic exposure compared with room air-exposed rats. [Conclusion] In the setting of moderate lung injury, the alveolar epithelial barrier is still capable of removing fluid at a normal arte.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第20期3110-3112,3115,共4页
China Journal of Modern Medicine
