摘要
目的探讨骨髓间充质干细胞(BMMSC)对异基因骨髓移植小鼠造血功能恢复的影响。方法将供鼠C57BL/6(H-2b)的骨髓细胞和体外培养的BMMSC联合输给致死量照射的受鼠BalB/c(H-2d)。在移植后第1、7、14天检测受鼠外周血红细胞(RBC)、白细胞(WBC)、血小板(BPC);移植后第7、14天检测受鼠骨髓中的脾集落形成单位(CFU-S)。结果联合移植组小鼠第7、14天的外周血红细胞、白细胞、血小板、CFU-S数均显著高于单纯移植组(P<0.01)。结论BMMSC能促进异基因骨髓移植后造血功能重建。
Objective To investigate the effect of co transfusion of bone marrow mesenchymal stem cells （BMMSC） on hematopoietic recovery in mice after allogeneic bone marrow transplantation （allo-BMT）. Methods Both BMMSC obtained after three to four weeks of culture and bone marrow cells of donor mice C57BL/6 （ H - 2^b ） were transplanted into the recipient mice BalB/c （ H - 2^d） that were lethally irradiated. Peripheral blood cells were counted on day 1,7, and 14 post-transplantation. CFU-S in recipient bone marrows were measured on day 7 and 14. Results The numbers of peripheral WBC, RBC, platelets and the number of CFU-S in recipient bone marrows on day 7 and 14 were all significantly higher in the co-infusion group than those in control group （ P 〈0. 01 ）. Conclusions BMMSC could improve the hematopoietic recovery after allo-BMT.
出处
《中国医师杂志》
CAS
2006年第10期1305-1306,共2页
Journal of Chinese Physician
关键词
间质干细胞
骨髓细胞
骨髓移植
移植
异种
造血系统
Mesenchymal stem cell
Bone marrow cells
Bone man＇ow transplantation
Transplantation, heterologous
Hematopoietic system
