摘要
目的:建立高效液相色谱法测定别嘌醇片含量的方法。方法:采用Shim-pack C18(150 mm×4．6 mm,5μm)分析柱,流动相为甲醇-水-冰醋酸(75:25:0．2),检测波长为251 nm;流速为1 ml·min-1。结果:别嘌醇浓度在8～72μg·ml-1范围内与峰面积呈良好的线性关系,r=0．999 9。平均回收率为99．3％,RSD为1．5％(n=9)。结论:本方法简便、快速、准确,可作为别嘌醇片的含量测定方法。
Objective： To establish a HPLC method for determination the content of allopurinol tablets. Method： The Shim-pack C18 column （ 150 mm × 4.6 mm , 5 μm ） was used, The mobile phase was methanol-water-glacial-acetic acid （ 75： 25： 0.2 ） , The detection wavelength was at 251 nm, The flow rate was 1 ml·min ^-1. Result： the linear range of allopurinol was 8-72 μg ·ml^-1 （ r = 0.9999 ）. The average recovery was 99.3% with RSD 1.5 % （n = 9 ）. Conclusion： This method is simple, rapid and accurate. It can be used for determination the content of allopurinol tablets.
出处
《中国药师》
CAS
2006年第11期993-994,共2页
China Pharmacist
