摘要
目的研究bc1-2蛋白在骨肉瘤中的表达,探讨与凋亡指数(AI)及预后的关系。方法应用免疫组化方法对34例骨肉瘤组织进行bcl-2蛋白的检测,应用细胞原位凋亡TUNEL技术检测细胞凋亡指数AI,并结合临床资料分析其对预后的影响。结果34例骨肉瘤中27例bcl-2蛋白(+)(79·5%)。bcl-2蛋白的表达与骨肉瘤分型无关,但与患者年龄、性别、5年生存状态相关,且与AI有相关性;另外,AI与预后有相关性。结论bcl-2蛋白、AI可能成为评估骨肉瘤预后的一项重要指标。
Objective To investigate the expression of bcl-2 protein in human osteosarcoma and its relationship to apoptotic index （AI） and prognosis. Methods The expression of bcl-2 protein and the AI were measured by the TUNEL and immunohistochemistry in 34 cases of osteosarcoma. Their relationship to prognosis was analyzed statistically. Results Twenty- seven of 34 osteosarcoma tissues were found positive staining of bcl-2 （79.5%）. The expression levels of bcl-2 were not related to Dahlin classification, but significantly related to in the 5 - year survival rate, age and gender of osteosarcoma. AI was related to and increased in the cases of living over 5 years and were significantly negative correlated to bcl-2. Conclusion The results suggest that bcl-2 and AI may serve as new biological indicators for the prognosis of patients with osteosarcoma.
出处
《诊断病理学杂志》
CSCD
2006年第5期365-367,I0014,共4页
Chinese Journal of Diagnostic Pathology
基金
国家自然科学基金资助项目(30371446)。
