摘要
目的探讨不同方式肺癌抗原负载的树突状细胞(DC)在抗肿瘤免疫中的作用。方法取肺癌患者外周静脉血体外诱导和培养DC,分别以肺癌细胞总RNA转染DC(转染组)、肺癌细胞融合DC(融合组)和肺癌细胞冻融抗原负载DC(冻融组),以未负载抗原的DC(未负载组)和T细胞组作为对照,比较各组(每组n=6)DC诱导的细胞毒性T淋巴细胞(CTL)对肺癌细胞A_(549)的杀伤率(%)。结果转染组、融合组、冻融组、未负载组和T细胞组的杀伤率分别为(73．2±5．9)%、(61．6±6．2)%、(55．3±6．9)%、(22．3±6．1)%和(19．8±6．3)%(P＜0．05)；其中转染组、融合组和冻融组高于未负载组和T细胞组(P＜0．05)；转染组和融合组之间差异无统计学意义(P＞0．05),但两者均大于冻融组(P＜0．05)。RNA转染DC所需的肿瘤细胞数仅是冻融及融合方式的1/5和1/6。结论3种肺癌抗原负载DC方式均有诱导效应细胞杀伤靶细胞的增效作用,而以肺癌细胞总RNA转染方式为佳。
Objective To compare 3 different methods of loading lung cancer antigen to dendritic cells （DCs） and to induce specific CTLs killing tumor cells. Methods DCs were induced and cultured from venous blood of lung cancer patients,then transfected with the total RNA of lung cancer cells （the transfection group）, merged with the lung cancer cells （the fused group）, or loaded by freeze-thawed lung cancer cells （the freeze-thawed group） respectively, and the cultured DCs and T lyrnphocytes served as control groups （ n = 6 in each group）, The rate of CTL killing As49 lung cancer cells was tested. Results （1） The killing rate of A549 was （73.2 ± 5.9） % in the transfection group, （61.6 ± 6.2） % in the fused group,（55.3±6.9）% in the freeze-thawed group, （22.3± 6.1）% in the DCs group and （19.8± 6.3） % in the T lymphocytes group respectively （P 〈 0.05）. The rate in the transfection and and the fused group was equal （P 〉 0.05）, but higher than that in the freeze-thawed group （ P 〈 0.05） ; （2） The number of tumor cells used in the transfeetion group was only 1/5 to the Fused group or 1/6 to the freeze-thawed group. Conclusion The response potency of the specific CTLs killing target cells may be increased by the total RNA of lung cancer cells transfeeting DCs,the lung cancer cells fusing DCs or the freeze-thawed lung cancer antigen loading DCs,and the total RNA transfecting DCs is optimal.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第11期1337-1339,共3页
Chinese Journal of Experimental Surgery
