摘要
目的探讨人乳头瘤病毒(HPV)16、18型感染与原发性非小细胞肺癌(NSCLC)组织中细胞增殖及凋亡的关系及其意义。方法应用PCR、TUNEL和免疫组化SP法分别检测76例NSCLC及13例肺良性病变中HPV16、18型DNA、细胞凋亡和增殖细胞核抗原(PCNA)的表达,并分别计算增殖指数(MI)和凋亡指数(AI)。结果NSCLC中HPV16、18型总阳性率为40.8%(31/76),而肺良性病变组阳性率为7.7%(1/13),差异有显著性意义(P<0.05)。HPV感染在不同组织学类型及有无淋巴结转移间差异无显著性意义,而在不同组织分化程度间差异有显著性意义(P<0.05)。NSCLC中HPV阳性组与阴性组之间MI及AI差异均有显著性意义(均P<0.05)。结论HPV16、18型感染在NSCLC发生中可能有病因学意义,其致癌机制之一可能通过减少癌变细胞凋亡和促进细胞异常增殖而导致细胞癌变。
Objective To investigate the relationship between HPV16, 18 infection and cell proliferation and apoptosis in non-small cell lung cancer and the significance. Methods PCR, TUNEL and immunohistochemistry were applied to detect HPV16, 18 DNA, cell apoptosis and PCNA expression in 76 NSCLC and 13 normal lung specimens, respectively. The mitotic index （MI） and apoptosis index （AI） were calculated. Results The positive rate of HPV16, 18 DNA in NSCLC and normal non-cancer groups was 40.8 % （31/76） and 7.7 % （1/13）, respectively （P〈0.05）. There was no statistically significant difference in HPV infection among histological types or lymph node metastases in NSCLC, but there was significant difference among differentiation degrees （P〈0.05）. There was statistically significant difference in AI and MI between HPV positive and negative groups in NSCLC （P〈0.05）. Conclusion HPV16, 18 infection may have etiologic significance for NSCLC. One of the carcinogenesis mechanisms may be contributed to the reduction of cancer cell apoptosis and promotion of cell abnormal proliferation.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期584-587,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省卫生厅资助项目(No.NX200504)
关键词
癌
非小细胞肺
人乳头瘤病毒
细胞增殖
细胞凋亡
carcinoma, non-small cell lung
human papillomavirus
cell proliferation
apoptosis
