期刊文献+

体外细胞机械牵张力学装置的研制及力学分布的有限元分析 被引量:9

Development of a mechanical stretching-loading device for cultivated cells
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摘要 [目的]建立一种干预体外细胞生长的周期性机械牵拉力学装置。[方法]使用微型电动机控制的支撑物上下位移运动,隆起6孔板具有硅胶弹性的底部基膜,发生周期性形变,使贴附其上的贴壁细胞受牵张应力,并利用三维有限元的方法分析基膜的力学分布,进一步将此装置应用到骨髓间充质干细胞(MSCs)的培养中,对系统进行验证。[结果]此装置操作简单,性能稳定,参数可调性强,生物相容性好,应力变化均匀连续,适当的牵张应力可刺激细胞增殖。[结论]模型可以用于体外应力刺激细胞效应的实验研究。 [ Objective] To develop a mechanical stretching-loading device for cell culture. [ Method] A mechanical stretching-loading device for cultivated cells was designed and installed. A bioflex for cell culture dish with a elastic, silicon botton membrane was placed on the support of device. The cell culture plate attached to botton membrane was lyelie stretched and deformed as the menbrane on the support moved up and down controlled by a controlablc micromotor. A three-dimension finite element analysis model was developed to analyze the strain and stress on elastic membrane which represent in turn that on the cultivated cells. Mesencymal stem cells (MSCs) were cultivated on this elastic membrane under a strain rate of 0% ( control group) , 4% (group B) , 6% ( group C) , 9% ( group D), and 12% ( group E) experimental groups. [Result] The device was operated easily and the parameters recoded was adjusted conviniently and reliablly in applying stretching-loading on euhivated cells. As comparison with control group, the cultivated MSCs grew better in a regular arrangement along the lengent direction of applied stress and its proliferation activity was significantly better in group D and group E. [ Conclusion] The new developed tension-loading unit operates easily and provides a reliable parameters of loading tension for cuhivated cells, and can be used to evaluate cell response sensibility to mechanical tension stress.
出处 《中国矫形外科杂志》 CAS CSCD 北大核心 2006年第21期1647-1650,I0003,共5页 Orthopedic Journal of China
基金 教育部回国人员基金项目(No.023456)
关键词 细胞 机械牵张 装置 有限元分析 mesencymal stem cells mechanical stretching-loading new device finite-element analysis
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参考文献8

  • 1Neal X,Chen,Derik J,et al.Fluid shear-induced NFx B translocation in osteoblasts is mediated by intracellular calcium release[J].Bone,2003,33 (3):399-410.
  • 2Mitsui N,Suzuki N,Koyama Y,et al.Effect of compressive force on the expression of MMPs,Pas,and their inhibitors in osteoblastic Saos-2cells[J].Life Sci,2006,1.
  • 3Takahiro K,Tetsu T,Wataru A,et al.Tensile mechanical strain upregulates Runx2 and osteogenic factor expression in human periosteal cells Implications for distraction osteogenesis[J].J Oral Maxillofac Surg,2005,63:499 -504.
  • 4Jonathan L,Schaffer,Michael R,et al.Device for the application of a dynamic biaxially uniform and isotropic strain to a flexible cell culture membrane[J].Journal of Orthopaedic Rueurch,1994,12:709-719.
  • 5Thomas D.Brown Techniques for mechanical stimulation of cells in vitro:a review[J].Journal of Biomechanics,2000,33:3-14.
  • 6黎润光,邵景范,魏明发.机械牵张应力对成骨细胞的影响研究进展[J].中国矫形外科杂志,2006,14(6):457-460. 被引量:12
  • 7Yamamoto N,Fukuda K,Matsushita T,et al.Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblas-like cells[J].Calcif Tissue Int,2005,76:433-438.
  • 8黄岂平.不同基底拉伸过程对细胞生长、取向的影响及其骨架重排机制探讨[D].重庆大学博士学位论文,2002,11

二级参考文献20

  • 1王鹏程,张英泽,陈百成,李东风.牵拉应力下成骨细胞(MC3T3-E1)的增殖与matrilin-2 mRNA的表达[J].中华创伤骨科杂志,2004,6(8):877-880. 被引量:4
  • 2戚孟春,胡静,韩立赤,邹淑娟,王大章.成骨细胞在机械力刺激下细胞骨架及细胞形态改变的体外研究[J].中国口腔颌面外科杂志,2004,2(3):181-184. 被引量:16
  • 3席雨涛,马爱群,耿涛,白晓君,吴格如.体外心肌细胞牵张刺激装置的建立[J].中国病理生理杂志,2004,20(11):2158-2160. 被引量:3
  • 4Thomas D.Brown.Techniques for mechanical stimulation of cells in vitro:a review[J].Journal of Biomechanics,2000,33:3-14.
  • 5Kaspar D,Seidle W,Neidlinger Wike C,et al.Proliferation of humanderived osteoblast-like cells depends on the cycle number and frequency of uniaxial strain[J].Biomech,2002,35:880-893.
  • 6Weyts F.A.A,Bosmans B,Niesing R,et al.Mechanical control of human osteoblast apoptosis and proliferation in relation to differen tiation calcif[J].tissue Int,2003,72:505-512.
  • 7Winter LC,Walboomers XF,Bumgardner JD.Intermittent versus continuous stretching effects on osteoblast-like cells in vitro[J].J Biomed Mater Res,2003,67:1269-1275.
  • 8Satoshi S,Hiroshi S,Katsuyo Y,et al.Mechanical stretching of human osteoblast-like cells stimulates bonemorphogenic proteins and macrophage colony-stimulating factor productions[J].Pathophysiology,1999,6:63-64、69.
  • 9Xuhui L,Xiaoliu Z,Zong-Ping L,et al.Strain-related collagen gene expression in human osteoblast-like cells[J].Cell Tissue Res.2005,17:1-4.
  • 10Fong KD,Nacamuli RP,Loboa EG,et al.Equibiaxial tensile strain affects calvarial osteoblast biology[J].J Craniofac Surg,2003,14 (3):348 -55.

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