摘要
目的:观察黄芪对大鼠视网膜缺血再灌注损伤的影响及其作用机制。方法:将90只大鼠随机分为3组:对照组、缺血组、保护组,采用前房灌注液体形成14.63kPa高眼压60min的方法建立模型,连续7d每天1次黄芪注射液6g/kg,ip,于手术前30min加注1次,对照组给予同等剂量的生理盐水。两组缺血60min后,分别再灌注0,2,6,12,24,48,72h,用比色法进行视网膜SOD、MDA、NO的测定,用光镜测量包埋切片的平均视网膜内层厚度。结果:黄芪能显著对抗大鼠视网膜缺血再灌注后MDA、NO水平的升高和SOD水平的降低,同时能改善平均视网膜内层厚度的变化。结论:黄芪可减轻大鼠视网膜缺血再灌注后的损伤,其机制可能与黄芪清除自由基,拮抗NO的毒性作用有关。
AIM： To investigate the effect of Astragalus membranaceus on retina in rats with the damage of retinal ischemia-reperfu- sion （RI R） and its mechanism.
METHODS： Ninety Wistar rats were divided randomly into three groups： control group, ischemia group and protective group. The intraocular pressure （IOP） was increased to 14.63KPa for 60 minutes with anterior chambers infused liquid to induce the models of retinal ischemia-reperfusion in rats. The intraperitoneal injection of Astragalus membranaceus at dose of 6g/kg was performed daily in consecutive 7 days and 30 minutes before operation to the rats in protective group, and the rats in ischemia group and control group received the intraperitoneal injection of sodium chloride with the same dose. At 1 hour after retinal ischemia, the intraperitoneal injection of sodium chloride was performed for 0, 2, 6, 12, 24, 48 and 72 hours after reperfusion to the rats in protective and ischemia groups. The effect of Astragalus membranaceus on retinal degeneration was assessed by measuring the level of SOD, MDA. NO and the thickness of the inner retinal layers （MTI RL）.
RESULTS： The level of SOD was decreased but the level of MDA and NO were both increased after ischemia-reperfusion in retina of rats. The changes of SOD, MDA and NO were blocked by Astragalus membranaceus.
CONCLUSION： Astragalus membranaceus can protect the retina from ischemia-reperfusion injury. The effects might be related with antagonizing the free radicals and NO.
出处
《国际眼科杂志》
CAS
2006年第5期1042-1044,共3页
International Eye Science
关键词
黄芪
视网膜
缺血再灌注
Astragalus membranaceus
retina
ischemia-reperfusion
