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全反式维甲酸抑制兔晶体后囊膜混浊实验观察

Prevention of posterior capsule opacification by retinoic acid in rabbit eyes
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摘要 目的研究全反式维甲酸对兔晶状体后囊膜混浊的抑制作用及有效抑制浓度。方法新西兰大白兔12只24只眼,分为右眼实验组(A组)和左眼对照组(B组),各12只眼。A组兔眼行白内障囊外摘除术(ECCE术),前房灌注平衡盐液(BSS)中加入5.63μg/ml或6.06μg/ml的全反式维甲酸,B组兔眼行ECCE术,BSS中不加入维甲酸。术后1月至术后4-4.5月观察结膜、角膜、前房炎症及后囊膜情况。术后4~4.5月取出兔眼标本,石腊包埋及切片后病检。结果术后第1~4天术眼有不同程度的结膜浅充血、睫状充血、角膜水肿、房水混浊,术后第5天角膜水肿、房水混浊明显好转,1周后结膜浅充血及睫状体充血消退,两组差异无显著性意义(P〉0.05);B组兔晶状体后囊膜梭形细胞及纤维细胞增生、后囊膜增厚明显高于A组(P〈0.005)。结论全反式维甲酸浓度为5.63μg/ml及6.06μg/ml对兔晶状体后囊膜混浊有抑制作用。 Objective To investigate the concentration-dependent inhibitory effect of all-trans retinoic acid (RA) in preventing posterior capsule opacification (PCO) in rabbit lens epithelial cells (RLEC) , and give a clue to the study on the prevention of PCO. Methods Twenty-four eyes from twelve rabbits were divided into two groups. Group A ( 12 right eyes ) was undertaken ECCE surgeries using BSS plus RA (5.63 or 6.06 μg/ml) , while group B ( 12 left eyes) had ECCE surgeries with BSS only and served as control, Slitlamp, ophthalmoloscopy and anterior segments were monitored for 4 to 4, 5 months following operation. Furthermore, proliferation of lens epithelial cells was evaluated histologically 4 to 4.5 months after surgery, Results Clinical assessments showed that RA treatment during surgery was very effective in preventing PCO compared with the untreated controis ( P 〈 0.005 ). Histological analysis also revealed a significant reduction in cell proliferative activity on posterior capsules in the RA-treated group in contrast to the appearance of muhilayered ceils in the control group, There was no obvious difference in cornea, anterior chamber, iris, ciliary body and cytological analysis of aqueous humor between two groups. Conclusion RA significantly prevents the development of PCO in rabbit eyes without obvious toxicity to the normal ceils in the anterior segment, Optimal biocompatible dosage must be carefully determined by further investigation.
出处 《实用医院临床杂志》 2006年第6期26-27,共2页 Practical Journal of Clinical Medicine
关键词 全反式维甲酸 后囊膜混浊 平衡盐液 兔晶状体上皮细胞 人晶状体上皮细胞 All-trans retinoic acid Posterior capsule opacification Balanced sodium solution Rabbit lens epithelial cells Human lens epithelial cells
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  • 1[1]Nasissi MP,Dykstra MJ,Cobo LM.Lens capsule opacification in aphakic and pseudophakic eyes[J].Graefes Arch Clin Exp Ophthalmol,1995,233:263.
  • 2[3]Mastropasqua L,Lobefalo L,Ciancalinni M,et al.Heparin eye drops to prevent posterior capsule opacification[J].J Cataract Refract Surg,1997,23:440.
  • 3夏小平,陆道炎.后发性白内障[J].国外医学(眼科学分册),1992,16(1):28-31. 被引量:60
  • 4[5]Nishi O.Incidence of posterior capsule opacification in eyes with and without posterior chamber intraocular lenses[J].J Cataract Refract Surg,1986,12:519.
  • 5[6]Roberts AB,Spom MB.Cellular biology and biochemistry of the retinoids on[M].Orlando:Academic Press Inc,1984.
  • 6[7]Doyle JW,Dowgigrt RK,Buzney.SM.Factors modulating the effect of retinoids on cultured retinal pigment epithelial cell prolifieration[J].Curr Eye Res,1992,11(8):753-765.
  • 7[8]Araiz JJ,Refoio MF,Arroy MH,et al.Antiproliferative effect of retinoic acid in intravitreous silicon oil in an animal modle of proliferative vitreiretinopathy[J].Invest Ophthalmol Vis Sci,1993,34:522.
  • 8栾洁,王文吉,陈钦元,赵培泉.维甲酸硅油防治实验性增殖性玻璃体视网膜病变[J].中华眼底病杂志,1997,13(3):174-176. 被引量:19
  • 9石磊,高殿文,柏春莉.全反式维甲酸预防后囊膜混浊的研究[J].中国公共卫生,2002,18(3):289-290. 被引量:1
  • 10[11]Inan UU,Ozturk F,Kaynak S,et al.Prevention of posterior capsule opacification by retinoic acid and mitomycin[J].Graefes Arch Clin Exp Ophthalmol,2001,239(9):693-697.

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