摘要
目的研究硒化紫球藻胞外粗多糖的理化性质。方法在ASW培养基中加入适量的亚硒酸制备硒化紫球藻胞外多糖,并通过DEAE-52树脂分离纯化,Sephadex G200层析法鉴定其纯度;硫酸苯酚法测定硒化紫球藻多糖溶解度;pH计测量其溶液酸度;紫外可见分光光度法于260nm和280nm处分别定性测量核酸及蛋白质;纸层析法分离多糖;琼脂糖凝胶电泳测定溶液中糖带电情况。结果与结论培养过程中加入适量亚硒酸,对紫球藻生长没有显著性影响;制备的PSP及Se-PSP均不含蛋白质和核酸,显酸性、带负电荷;可能含有两个组分等。
Objective To study the physiochemical properties of selenium exopolysaccharide from Porphyridium sp. （Se-PSP）. Methods The Se-PSP was obtained from ASW,a sort of culture medium added with some selenious acid solution,and was purified by DEAE-52 cellu lose column and identified by Sephadex G-200. The acidity ,UV spectral absorption, paper chromatography, and gelose electrophoresis of Se-PSP solution were studied. Results and Conclusion Proper concentration of selenious acid in ASW culture had no influences upon the growth of Porphyridiurn. Se-PSP prepared in this way didn＇t contain protein and nucleic acid and the solution of Se-PSP showed acidity. It is probable that Se-PSP contain two fractions of PSP.
出处
《中国海洋药物》
CAS
CSCD
2006年第4期34-36,共3页
Chinese Journal of Marine Drugs
关键词
硒
紫球藻
胞外多糖
selenium
Porphyridium sp.
exopolysaccharide
