摘要
目的 建立能长期存活的山羊心肌梗死实验模型。方法 16只黑山羊，体重15～24kg，3～6月龄，雌雄不拘。均行剑突下切口，倒V字形切开胸骨下段，于左冠状动脉前降支中下1／3用5-0prolene线8字缝合包绕伴行静脉，阻断5min，再间歇3min，重复两次进行缺血预适应，然后结扎左冠状动脉及伴行静脉，建立羊心肌梗死模型。描记心电图，于建模前及建模后1、3、6、12、24、48h抽血检测血清肌酸激酶同工酶（creatine kinase—MB，CK—MB）及脂肪酸结合蛋白；并于模型建立后6周行心电图、超声心动图、选择性左冠状动脉造影、透射电镜及心肌梗死面积测定。结果 造模过程中无实验动物死亡，造模成功率为100％。CK—MB于建模后3h开始升高，12h达高峰；脂肪酸结合蛋白于建模后1h开始升高，12h达高峰。建模后结扎点下方心肌逐渐出现片状紫红色区域，心电监测仪提示Ⅱ、Ⅲ、V3导联明显ST段上抬及T波改变，建模后6周，相应导联均出现明显病理性Q波；心脏超声检查显示左心室腔明显扩大并见室壁瘤形成；选择性左冠状动脉造影检查见心尖区血管纤细，外形呈瘤样扩张。透射电镜见梗死区肌丝溶解、断裂，排列混乱，细胞器崩解聚集。心肌梗死面积为4．74±0．78cm^2。结论 黑山羊是建立心肌梗死模型较好的实验动物；经剑突下切口，倒V字形切开胸骨下端入路，结扎左冠状动脉前降支中下1／3，是建立能长期存活的陈旧性心肌梗死模型简便、安全可靠的方法。
Objective To establish an effective model of myocardial infarction in black goat so as to provide a safe, convenient and credible model of myocardial infarction for treatment and research. Methods Sixteen black goats were made chronic myocardial infarction by ligation of far end of left anterior descending coronary artery through incision below xiphoidprocess. Electrocardiogram （ECG） and serum myocardial enzymes were investigated before and after occlusion. Echocardiographic measurements were performed, and left coronary artery angiography was performed with digital subtraction angiography （DSA） before infarction and 6 weeks after infarction. The myocardial uhrastructure were observed. Results All goats survived more than 6 weeks. ECG showed ambulatory change, ST-segment elevated half an hour after occlusion and pathologic Q waves 6 weeks after infarction, CK-MB significantly increased. Echocardiographic indexes showed significant decrease of maximal peak A, percent wall thickening（WHT） and ejecting fraction （EF）, increase of end-systolic volume （ESV）, end-diastolic volume （EDV）, and dilation of left ventricle. DSA showed block or decrease of perfusion of far end of left anterior descending coronary artery. Conclusion It is safe, convenient and credible to establish model of myocardial infarction by ligation of far end of left anterior descending coronary artery through incision below xiphoidprocess in black goat.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第11期1124-1129,共6页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
陈旧性心肌梗死
动物模型
黑山羊
Chronic myocardial infarction Animal models Black goat
