摘要
目的:复制不同类型燃煤型氟骨症动物模型。方法:以SD大鼠为研究对象,按体重均衡随机分为五组(组内雌雄各半);高氟组、高氟偏食组、低氟组、低氟偏食组、对照组。以氟病区煤烘玉米为主要饲料,复制氟骨症动物模型。以股动脉放血法处死动物,查看氟斑牙、测定尿氟、牙氟、骨氟、骨密度(BMD)。对其胫骨干骺端进行常规组织切片,HE染色观察组织学改变。结果:高氟、低氟组大鼠骨密度增高,与对照组比较差异具有统计学意义(P<0.05),胫骨病理显示成骨活跃。高氟、低氟偏食组大鼠骨密度降低,其中,高氟偏食组与对照组比较差异具有统计学意义(P<0.05),胫骨病理显示高氟偏食组大鼠密质骨呈骨软化,低氟偏食组呈轻度疏松化。结论:过量氟造成氟骨症大鼠成骨与破骨活动均增强的高骨转换状态,低钙偏食可使氟骨症破骨性骨吸收增强。
Objective： To establish the animal model of skeletal fluorosis caused by coal- burning. Methods： SD rats taken as the experimental objects were randomly assorted into 5 groups （the number either female or male was the same in each group） ： control group, high-dose fluorine group, high- dose fluorine supplemented with low calcium diet group, low- dose fluorine group, low- dose fluorine supplemented with low calcium diet group. All rats were fed with corn dried by burning coal, which came from disease borough with high fluorine, to establish the animal model of skeletal fluorosis. All rats were killed by femoral arterial exsanguination, followed by examinations of dental fluorosis, fluorine in urine, bone and dental, bone mineral density （BMD）. The tibia slices of chronic fluorosis rats were stained with HE. Results： Compared with control group, the BMD increased except that in high - dose fluorine supplemented with low calcium diet group , which was statistically significant （P 〈 0.05）. The enhanced osteoblastic activity was found from the tibia slices. The BMD in high- dose fluorine supplemented with low calcium diet group was lower than that in control group （P 〈 0.05）. The compact bone of the cortex in high- dose fluorine supplemented with low calcium diet group became intenerate while it become porosity in low-dose fluoride supplemented with low calcium diet group. Conclusion： Excessive fluorine can induce accelerative bone turnover by stimulatinging the activity both osteoblasts and osteoclasts, Low calcium diet can promote bone absorption of skeletal fluorosis.
出处
《现代预防医学》
CAS
北大核心
2006年第11期2100-2102,共3页
Modern Preventive Medicine
基金
贵州省科技基金重点资助项目(黔基金[2002]3011)
关键词
煤
氟骨症
动物模型
复制
Coal
skeletal fluorosis
animal model
Replication
