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黄花菜的离体培养中胚状体的发生和再生植株形成的研究 被引量:13

A Study on Embryoid Development and Establishment of Plantlets From the Explants of Hemenocalis Citrina Bar.
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摘要 将黄花菜的幼嫩花梗及花托培养在N6+2.4-D2+6-BA1培养基上,已经诱导出大量胚状体,在相同培养基上继代培养,建立了黄花菜胚状体元性系.成熟的胚状体,转到1/2MS+NAA0.2培养基上,长成了带有根系的完整植株.再生植株成功地移栽到土壤基质中,成活率达90%以上. A number of embryoids were induced with young floral axis and petal explants of Hemenocallis citrina Bar. cultuled on N6 medium condoning 2ppm 2. 4-D and 1ppm BA. After stepculture, a clone of elnbryoids were formed in the same cultural medium. the mature embryoids cultured on 1/2 MS medium containing 0. 2ppm NAA, have grown up to plantlets with root system. The plantlets were then transfered to soil condition and 90% of them were viable.
出处 《海南大学学报(自然科学版)》 1996年第4期321-324,共4页 Natural Science Journal of Hainan University
关键词 黄花菜 胚状体 再生植株 离体培养 发生 hemenocallis citrina Bar embryoid regenerated plant
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参考文献2

  • 1周朴华,胡继金,范鸿芝.从黄花菜诱导出胚状体并发育成植株的研究[J]园艺学报,1983(04).
  • 2黄纯真.应用组织培养方法获得四种花木试管植物的初步试验[J]植物生理学通讯,1980(06).

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