摘要
A total of 85 interspecific hybrid F2 (Cyprinus carpio var.wuyuanensis ×Cyprinus pellegrini pellegrini) were cooled to specific temperatures and held at those temperatures over a maximum of 4 days in a water-recycled and temperature-controlled aquarium inside.As a result,the blood homeostasis of experimental fish changed violently as acute temperature changed from 16 ℃ to 10 ℃ and 4 ℃ at a rate of 1 ℃·h-1 according to the data we collected.Whole blood pH,also called extracellular pH(pHe) were very sensitive to temperature changes,where there was a significant difference between 10 ℃(7.41) and 16 ℃(7.17)(P<0.01),compared to other values of hematology and serum chemistry.When the water temperature was continually decreased to an extreme temperature of 4 ℃,the content of Na+ of serum decreased remarkably in comparison with that of 10 ℃ and 16 ℃,which was 85.2 mmol·L-1,113.3 mmol·L-1 and 118.7 mmol·L-1,respectively.The values of hematology and serum chemistry also altered in gentle temperature changes of(10±2) ℃ and(4±2) ℃.Most values of serum chemistry and pH changed significantly,whereas the values of blood plasma changed slightly.pH was up slowly in 4 days at(10±2) ℃ and down slowly in 3 days at(4±2) ℃.A variety of values of serum chemistry changed remarkably both at(10±2) ℃ and(10±2) ℃,but the values of TP,TG and ALB only changed significantly at(4±2) ℃.These results distinguished at least two mechanisms involved in cold-induced stress in hybrid F2.Cold-induced pH changes resulted in other values altered.What’s more,pH correlated negatively with water temperature above 10 ℃,and the content of Na+.We also found that gentle temperature changes will be physiologically compensated for on day one at(10±2) ℃ and on day 2 at(4±2) ℃ in hybrid F2.
A total of 85 interspecific hybrid F2 ( Cyprinus carpio var. wuyuanensis × Cyprinus pellegrini pellegrini ) were cooled to specific temperatures and held at those temperatures over a maximum of 4 days in a water-recycled and temperature-controlled aquarium inside. As a result, the blood homeostasis of experimental fish changed violently as acute temperature changed from 16 ℃ to 10 ℃ and 4℃ at a rate of 1 ℃ -h^-1 according to the data we collected. Whole blood pH, also called extracellular pH (pile) were very sensitive to temperature changes, where there was a significant difference between 10 ℃(7.41 ) and 16℃ (7.17 ) ( P 〈 0. 01 ), compared to other values of hematology and serum chemistry. When the water temperature was continually decreased to an extreme temperature of 4 ℃, the content of Na^± of serum decreased remarkably in comparison with that of 10℃ and 16℃, which was 85. 2 mmol·L^-1, 113.3 mmol·L^-1 and 118.7 mmol·L^-1, respectively. The values of hematology and serum chemistry also altered in gentle temperature changes of ( 10 ± 2) ℃ and (4 ±2) ℃. Most values of serum chemistry and pH changed significantly, whereas the values of blood plasma changed slightly, pH was up slowly in 4 days at ( 10 ± 2) ℃and down slowly in 3 days at (4 ± 2)℃. A variety of values of serum chemistry changed remarkably both at ( 10 ± 2) ℃ and ( 10 ± 2)℃, but the values of TP, TG and ALB only changed significantly at (4 ± 2)℃. These results distinguished at least two mechanisms involved in cold-induced stress in hybrid F2. Cold-induced pH changes resulted in other values altered. What's more, pH correlated negatively with water temperature above 10 ℃, and the content of Na^± . We also found that gentle temperature changes will be physiologically compensated for on day one at (10±2) ℃and on day 2 at (4± 2) ℃ in hybrid F2.
出处
《水产学报》
CAS
CSCD
北大核心
2006年第5期701-706,共6页
Journal of Fisheries of China
基金
国家"973"计划(2004CB117404)
关键词
鲤
低温
胁迫
血液pH
Cyprinus carpio
cooling temperature
stress
pH