摘要
采用BSA法对垦农18(高油,23.21%)与黑农小粒豆(低油,19.0%)的杂交F2:3群体进行RAPD分析。用200个随机引物对构建的高油DNA池和低油DNA池进行扩增,有46个引物产生了RAPD扩增产物,共获得315个片断,其中产生多态性片段的随机引物为4个,同高油含量相关的特征性条带分别为S56-900bp、S61-600bp、S107-600/450/370bp、S134-450/500bp。这些标记平均可以区分F2:3群体中87.1%的高油与低油单株,重复性好,可以用作高油含量的分子标记。
With Bulked Segregation Analysis , RAPD method was employed to analyze genome DNA of F2：3 population obtained by crossing Kennong 18（oil content, 23.21 %） with Heinongxiaolidou（oil content, 19. 0%）. Two hundred random primers were used to amplify between the high and low oil content DNA pools, among which there were 315 amplified fragments by 46 primers that produced RAPD product and we found there were 4 random primers produced polymorphic bands. 4 markers related with oil content were Ss6-900bp, S61-600bp, S107-600/450/ 370bp,S134-450/500bp respectively. These markers could separate 87. 1% high or low plants in F2：3 population. The results showed very stable and repeatable. The RAPD markers was useful to assistant the selection for high oil content soybean plants in soybean hybrid breeding.
出处
《黑龙江农业科学》
2006年第6期6-8,共3页
Heilongjiang Agricultural Sciences
基金
黑龙江省青年基金项目(QC04C34)
关键词
高油大豆
RAPD
分子标记
RAPD
molecular marker
oil content
soybean
