摘要
株高是水稻重要的农艺形状之一,植株过高将导致倒伏和减产,目前,很多新的技术究被用来鉴定,图位克隆与水稻株高相关的基因及机理的研究,本实验选择优质早籼稻佳禾早占种植苗和经过组培获得的矮化突变水稻为材料,为研究比较它们间的遗传物质上差异,根据康耐尔大学的资料设计了311对SSR引物对佳禾早占种植材料和组培材料进行分析,对两种材料进行PCR多态性扩增,结果发现两者间存在多态性的引物有88对,多态性比例达到30.3%。在矮杆材料中不但验证了已报道的11个与调控株高性状基因相连锁的标记连锁群,同时在第3号染色体和第9号染色体上还获得了两个以前基本未有报道的标记集中分布区域。结果说明,该培养基培育出的佳禾早占水稻后代所表现出的矮杆性状与亲本在遗传物质上确有明显差别。该结果有助于挖掘和定位新的矮杆基因,并有利于今后在水稻育种中进行水稻株高性状的控制,同时也为开展矮化性状机理的研究提供有利的实验材料。
Plant height is one of the important agronomic traits of rice. Overhigh plant easily led to lodge and reduce output. Recently, great advances have been made in the identification, mapping, cloning and action mechanism of plant height genes in rice. This present paper selected the tissue cultured and cultivated paddy rice of Jia He Zao Zan （J. H. Z. Z） as experimental object, which were significant different on the height, the tissue cultured plant was only less than half height of the cultivated plant. In order to compare their differences of genetic and determine whether there are relationship between the dwarf gene and the dwarf character of tissue cultured plant, Polymorphism analysis was conducted between them by 311 pairs of rice SSR primers, designed by Cornell University, the result indicated that 88 pairs of primers had polymorphism between two kinds of materials, the ration in total primers was about 30. 3%, the experimental result not only verified 11 multiple groups which regulate the height gene, but also obtained two group regions which control the dwarf character on chromosome 3 and chromosome 9, which have not been reported previously, these results will help to explore and determine the situation of new dwarf gene, and will be of benefit to act genetic manipulation of plant height of in rice breeding. These results also provided the evidence for studying the mechanism of dwarf.
出处
《植物研究》
CAS
CSCD
北大核心
2006年第6期703-707,共5页
Bulletin of Botanical Research
基金
国家863项目(2002AA211091)
(20001AA11091)
厦门大学细胞生物学和肿瘤细胞工程教育部重点实验室开放基金资助(NO:2005108)资助
