摘要
目的探讨全反式维甲酸及胆酸钠作用于人卵巢癌细胞株时对细胞内核因子κB(NF-κB)表达的影响。方法对经全反式维甲酸、胆酸钠及两者联合干预COC2细胞后不同时间点的细胞核NF-κB进行提取,采用电泳迁移率变动分析法对细胞核内NF-κB含量进行分析。结果全反式维甲酸组癌细胞在用药24h内随作用时间的延长出现NF-κB活性上升趋势,而胆酸钠能抑制癌细胞的NF-κB活性,两者联合应用较单独应用全反式维甲酸时能增强抗癌作用并且降低细胞内NF-κB活性。结论全反式维甲酸及胆酸钠联合应用能降低对癌细胞NF-κB的激活作用,从而减少耐药性产生。
Objective To study the expressions of nuclear factor kappa B（NF-κB） in epithelial ovarian cancer cells treated with all-trans retinoic acid（ATRA） and/or bile acid sodium（SBA）. Methods COC2 cells treated with different drugs were divided into group ATRA, group SBA and group ATRA＋SBA. NF-κB of cells nuclei was determined by electrophoretic mobility shift assay （EMSA） after drug-challenged for 3, 6, 12, 24 and 48 hours. Results The expression of NF-κB in group ATRA was increasing significantly and time-dependently within 24 hours, which was suppressed by SBA. Conclusion The use of ATRA combined with SBA can reduce NF-κB activation and ATRA-induced drug-resistant effect.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第11期1028-1030,共3页
Jiangsu Medical Journal
关键词
全反式维甲酸
胆酸钠
核因子ΚB
卵巢癌
All-trans retinoic acid
Bile acid sodium
Nuclear factor kappa B
Ovarian cancer
