摘要
应用地高辛标记的Ｐ￣(５３)、ｃ－ｍｙｃｃＤＮＡ探针对２１例胆囊癌石腊切片上的ｍＲＮＡ进行原泣杂交。结果表明ｃ－ｍｙｃ和Ｐ￣(５３)ｍＲＮＡ的表达均位于细胞核内。ｃ－ｍｙｃ和Ｐ￣(５３)ｍＲＮＡ在胆囊癌的表达率分别为６６．６％和５７．１％，在癌周正常组织中没有ｃ－ｍｙｃ、Ｐ￣(５３)ｍＲＮＡ的表达。ｃ－ｍｙｃ、Ｐ￣(５３)ｍＲＮＡ表达的强度和胆囊癌的原发肿瘤大小，有无淋巴结转移及远处转移有关。分期越晚，表达率越高。有淋巴结转移者高于无转移者。Ｐ￣(５３)ｍＲＮＡ表达的阳性率和胆囊癌的分化程度无关；而ｃ－ｍｙｃｍＲＮＡ的表达和分化程度有关，分化越差，表达率越高。
Digoxigenin-labelled P53and c-myc cDNA probes were used to study gallbladder carcinoma by in situhybridization on the paraffin sections for mRNA expression in 21 cases.The resillts were as follows:c-myc and P53mRNAexpressiOn were both located in the nuclei.The positive incidences of expression of both c-myc and P53mRNA in gallbladderwere 66.6%and 5 7.1%respectivelyNo expression in the normal tissue surrounding the carcinoma.The strength of theexpression of c-myc and P53mRNA in gallbladder were related to the size of primary tumors,metastasis of lymph node anddistance metastasis,However,there was no relationship between the expression of p'mRNA and the degree of carcinoma celldifferentiation but c-myc mRNA.
关键词
c-mycmRNA
胆囊癌
原位杂交
P53 mRNA c-myc mRNA Gallbladder carcinoma In situ hybridization
