摘要
以紫叶黄栌的当年生嫩枝茎段为外植体进行簇生芽诱导,研究结果表明,诱导簇生芽时,在MS +6-BA2．0 mg／L+NAA0．2 mg／L培养基上的诱导效果较好,分化率达100％;继代培养时,以MS+ 6-BA1．0 mg／L+KT 0．5 mg／L+NAA0．1 mg／L培养基增殖倍数较高,达到11．8倍;再生苗在1/2MS +NAA0．1 mg／L的培养基上生长良好;在炼苗中,移栽基质以蛭石+森林土较好,可促进小苗后期生长。
The tender stem of the current year of Cotins coggygria var. purpurens is used explant to induce cluster shoots. The research found out that the induced shoots on the medium of MS＋6 BA2.0 mg/ L ＋ NAA 0.2 mg/l. performed well, with differentiation rate of shoots at 100%. The propagation speed is up to 11.8 limes of general in secondary culture on the medium of MS＋6-BA1.0 mg/l. ＋KT0.5 mg/ L ＋ NAA 0.1 mg/L. The rooting of regenerated stem works better in the medium of 1/2MS ＋ NAA0. 1 mg/L. The medium of vermiculite mixed with wood soil in one to one as substratum better for trans planting in acclimatization stage.
出处
《陕西林业科技》
2006年第3期1-4,共4页
Shaanxi Forest Science and Technology
关键词
紫叶黄栌
组织培养
培养基
炼苗
Cotinus coggygria
tissue culture
culture medium
acclimatization
