摘要
目的采用反相高效液相色谱（RP-HPLC）法测定车前草中主要成分乌索酸和齐墩果酸的含量。方法采用高效液相色谱法。使用Nova-Pak C18柱（3．9mm×300mm，4μm），流动相为甲醇：水（88：12，V/V），流速为0.8ml／min，检测波长为210nm，柱温25℃。结果乌索酸在0．456～4．104μg范围内呈良好的线性关系（r=0．9997），平均回收率为98．2％，RSD=1．1％；齐墩果酸在0．156～1．384μg范围内呈良好的线性关系（r=0．9996），平均回收率为101．6％，RSD=1．7％。结论方法灵敏、准确、专属性强，重现性好，可作为车前草药材的质量控制方法。
Objective To establish a method for determining ursolic acid and oleanolic acid in Plantago asiatica L. by high performance liquid chromatography. Methods The separation was carried out on Nova- Pak C18 column （3.9 mm× 300 mm, 4 μm） with methanol -water （88：12,V/V） as the mobile phase,at a flow rate of 0.8 ml/min. The detection wavelength was set at 210 nm. The column was used at 25 ℃. Results Ursolic acid showed a good linear relationship in the range of 0.456 -4. 104 μg （r = 0.9997） and the average recovery was 98.2 %, RSD was 1.1% . Oleanolic acid showed a good linear relationship in the range of 0. 156 -1.384 μg （r =0.999 6） and the average recovery was 101.6 %, RSD was 1.7%. Conclusion The method is sensitive,accurate and specific with a good repeatability and can be used for the quality control of Plantago asiatica L.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第11期2119-2120,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家"863"计划重点资助项目(No.2002AA2Z3217)
