期刊文献+

供者自然杀伤细胞减轻GVHD并提高移植成活率的实验研究 被引量:2

Experimental Study of Donor Natural Killer Cells Reducing Graft-versus-host Disease and Enhancing Engraftment
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摘要 本研究观察激活的供、受者NK细胞能否减轻骨髓移植后GVHD和增加移植成活率。以C57BL/6小鼠作为供者,将接受6.5Gy60COγ射线全身照射的BALB/c受鼠随机分为4组,对照1组(无任何处理)、对照2组(骨髓移植)、实验1组(骨髓移植同时输注激活的受者NK细胞)和实验2组(骨髓移植同时输注激活的供者NK细胞),观察每组小鼠的活存期、嵌合率、GVHD临床和病理改变。结果表明:对照1组全部活存,实验1组比对照2组活存期短(P<0.05),实验2组比对照2组活存期延长(P<0.01)。实验1组GVHD临床及病理评分高于对照2组(P<0.05),实验2组GVHD临床及病理评分明显低于对照2组(P<0.01)。实验1组和实验2组嵌合率均较对照2组高(P<0.05),实验2组的嵌合率比实验1组明显增加(P<0.01)。结论:激活的供者NK细胞能延长实验小鼠的活存期,降低GVHD严重程度,增加移植成活率;而激活的受者NK细胞却缩短实验小鼠的活存期,加重GVHD严重程度。受者NK细胞也增加移植成活率,但比供者NK细胞作用小。 The purpose of this study was to investigate whether pretransplant infusion of reactive natural killer cells (NK cells)from donor or recipient can reduce graft-versus-host disease(GVHD) and enhance engraftment in bone marrow transplantation(BMT). Recipient BALB/c mice were divided into 4 groups after received 6.5 Gy total-body irradiation (TBI) : control group 1 was treated with nothing, control group 2 received BMT alone, experiment group 1 received BMT and autoreactive NK cells, experiment group 2 received BMT and alloreactive NK cells. Life span, clinical and pathologic changes of GVHD and chimerism rate of each group were evaluated. The results showed that all mice were survival in control group 1. The life span was shorter in experiment group 1 than that in control group 2 (P 〈0.05)and longer in experiment group 2 than that in control group 2( P 〈0.01 ). GVHD was higher in score of experiment group 1 than in control group 2 ( P 〈0.05)but lower in experiment group 2 than that in control group 2( P 〈0.01 ). The donor chimerism rate in both two experiment groups were higher than that in control group 2 (P 〈 0.05 ), however, the donor chirnerism rate was higher in experiment group 2 than that in experiment group 1 ( P 〈 0.01 ). It is concluded that pretransplant infusion of alloreactive donor NK cells can prolong life span, reduce the degree of GVHD and enhance engrafanent. But autoreactive recipient NK cells can shorten life span, aggravate the degree of GVHD and also enhance engraftment, which is weaker than that using alloreactive donor NK cells.
出处 《中国实验血液学杂志》 CAS CSCD 2006年第5期924-927,共4页 Journal of Experimental Hematology
关键词 骨髓移植 NK细胞 GVHD 移植成活率 bone marrow transplantation natural killer cell GVHD engraftment
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参考文献7

  • 1Davies SM,Ruggieri L,DeFor T,et al.Evaluation of KIR ligand incompatibility in mismatched unrelated donor hematopoietic transplants.Blood,2002; 100:3825-3827
  • 2Ruggeri L,Capanni M,Urbani E,et al.Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants.Science,2002; 295:2097-2100
  • 3段连宁,郭坤元,袁进,杜江,王三斌,张立成,李玉华.同种异基因Th2细胞移植对GVHD和GVL效应的作用[J].中国实验血液学杂志,2000,8(1):57-60. 被引量:7
  • 4Bryson JS,Jennings CD,Lowery DM,et al.Rejection of an MHC class Ⅱ negative tumor following induction of murine syngeneic graft-versus-host disease.Bone Marrow Transplant,1999; 23:363-72
  • 5Cooke KR,Kobzik L,Martin TR,et al.An experimental model of idiopathic pneumonia syndrome after bone marrow transplantation:I.the roles of minor H antigens and endotoxin.Blood,1996; 8:3230-3239
  • 6Young NT.Immunobiology of natural killer lymphocytes in transplantation.Transplantation,2004; 15,78:1-6
  • 7Cooper MA,Fehniger TA,Fuchs A,et al.Nk cell and DC interactions.Trends Immunol,2004; 25:47-52

二级参考文献8

  • 1王江滨,王育珊.IL-4在诱导CD4,45RA^+和CD4,45RO^+T细胞向IL-4产生细胞分化过程中的效应[J].中国免疫学杂志,1997,13(1):10-14. 被引量:4
  • 2KrengerW,HillGR,FerraraJL.Cytokinecascadesinacutegraft-versus-hostdisease.Transplantation,1997;64:553-558
  • 3UharekL,ZeisM,HartungG,etal.CD4+donorTcellsfortheinductionofgraft-versus-leukemiaeffectafterstemcelltransplantation.BoneMarrowTransplant,1998;21(S1):Abstract240
  • 4KrengerW,SnyderKM,ByonJC,etal.Polarizedtype2alloreactiveCD4+andCD8+donorTcellsfailtoinduceexperimentalacutegraft-versus-hostdisease.JImmunol,1995;155:585-593
  • 5BlazarBR,TaylorPA,Panoskahsis-MortariA,etal.Rapamycininhibitsthegenerationofgraft-versus-hostdisease-andgraft-versus-leukemia-causingTcellsbyinterferingwiththeproductionofTh1orTh1cytotoxiccytokines.JImmunol,1998;160:5355-5365
  • 6TanakaJ,ImamuraM,KasaiM,etal.Theimportantbalancebetweencytokinesderivedfromtype1andtype2helperTcellsinthecontrolofgraft-versus-hostdisease.BoneMarrowTransplant,1997;19:571-576
  • 7FowlerDH,KurasawaK,SmithR,etal.DonorCD4-enrichedcellsofTh2cytokinephenotyperegulategraft-versus-host-diseasewithoutimpairingallogeneicengraftmentinsublethallyirradiatedmice.Blood,1994;84:3540-3549
  • 8刘荷中,毛宁.移植物抗宿主病研究的进展[J].国外医学(输血及血液学分册),1999,22(1):22-26. 被引量:8

共引文献6

同被引文献20

  • 1李晓红,高春记.异基因造血干细胞移植中NK细胞的作用[J].中国实验血液学杂志,2006,14(4):845-848. 被引量:8
  • 2曾慧,何广胜,吴德沛.树突状细胞与移植免疫耐受[J].中国实验血液学杂志,2006,14(4):849-852. 被引量:6
  • 3Carella AM , Champlin R, Slavin S, et al. Mini-allografts : ongoing trials in humans. Bone Marrow Transplant, 2000; 25:345 -350.
  • 4Bregni M, Dodero A, Peccatori J, et al. Nonmyeloablative conditioning followed by hematopoietic cell allografting and donor lympho- cyte infusions for patients with metastatic renal and breast cancer. Blood, 2002 ; 99:4234 - 4236.
  • 5Carella AM. Autografting and nonmyeloablative allogeneic stem cell transplantation in metastatic breast cancer. Hematologica, 2002; 87: 11 - 12.
  • 6Peccatori J, Ciceri F, Bemardi M, et al. Evidence of allogeneic graft-versus-tumor effect in prostate and ovarian cancer. Hematologica, 2002;87:12 - 14.
  • 7Childs R, Chernoff A, Contentin N, et al. Regression of metastatic renal cell carcinoma after nonmyeloablative allogeneic peripheral- blood stem cell transplantation. N Engl J Med, 2000; 343:750 - 758.
  • 8Peggs KS, Maekinnon S. Cellular therapy: donor lymphocyte infusion. Curr Opin Hematol, 2001 ;8:349 -354.
  • 9Gilleece MH, Dazzi F. Donor lymphocyte infusions for patients who relapse after allogeneic stem cell transplantation for chronic myeloid leukaemia. Leuk Lymphoma, 2003 ;44:23 - 28.
  • 10Hale DA, Gottschalk R, Umemura A, et al. Immunologic mechanisms in tolerance produced in mice with nonradiation-based lymphoablation and donor-specific bone marrow. Transplantation, 2002 ; 74 : 477 - 484.

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