摘要
目的应用白三烯受体1拮抗剂孟鲁斯特(montelukast,MK)治疗小鼠急性哮喘呼吸道炎症,探讨MK对白细胞介素-5(IL-5)和嗜酸性粒细胞趋化因子(eotaxin)表达及血清总IgE水平的影响。方法卵清蛋白(OVA)致敏并激发Balb/c小鼠建立急性哮喘模型。静脉予MK(25 mg/kg)或Saline 3 d。瑞氏染色观察小鼠支气管肺泡灌洗液(BALF)炎症细胞,酶联免疫吸附法(ELISA)检测肺组织、BALF和血清IL-5和eotaxin及血清总IgE水平。半定量逆转录聚合酶链式反应(RT-PCR)检测IL-5和eotaxin的mRNA表达。液相色谱方法检测血MK水平。结果OVA激发后24 h,MK组BALF白细胞总数、嗜中性和嗜酸性粒细胞(EOS)分别为(26.0±18.9)×107/L(、5.92±8.09)×107/L和(0.74±0.88)×107/L,较Saline组相应数明显减少(P均<0.05)。EOS减少达80%以上。MK组BALF和肺组织IL-5分别为(48.52±14.45)ng/L和(27.40±9.62)ng/g蛋白,较Saline组相应值明显降低(P均<0.05);MK组血清IL-5和总IgE水平明显减低。MK组BALF中eotaxin水平也明显降低(P<0.05);MK组肺组织IL-5 mRNA显著降低,肺eotaxin及其mRNA水平无明显下降。结论MK可能通过抑制IL-5和IgE产生和分泌起抗炎作用。
Objective To investigate the effects of montelukast（MK） ,a selective cysteinyl leukotriene receptor 1 antagonist on inter- leukin（ IL）- 5 and eotaxin expression as well as serum total immunoglobulin E（IgE） production during MK treatment of mouse acute asthma airway inflammation. Methods Sensitized Balb/c mice were challenged by ovabumin（OVA）to establish the acute asthmatic model. Twenty- five mg/kg of MK（MK group） or Saline （Saline group）were given by intravenously for 3 days. Cellular infiltration of bronchoslveolar larvage fluid （BALF） were assessed by Wright staining. Production of IL - 5 and eotaxin in the lung or BALF and serum IgE were determined by enzyme linked immunoeorbent assay （ELISA）. The expression of IL - 5 and eotaxin mRNA weremeasured by semi - quantified reverse transcriptase- polymerase chain reaction （RT- PCR）. Plasma MK level was determined by liquid chromatography. Results After 24 h OVA challenge,the numbers of total white blood cells, neutrophils and eosinophils （EOS）of BALF were（26.0 ± 18.9） × 10^7/L, （5.92 ± 8.09） × 10^7/L and （0.74 ± 0.88） × 10^7/L in MK treatment group. They were significantly reduced compared with those in Saline group, respectively （P 〈 0.05 ）. MK reduced the number of EOS by 〉 80 %. The level of IL - 5 in BALF and lung tissue were （48.52 ± 14.45） ng/L and （27.40 ± 9.62） ng/g protein in MK group, which significantly declined compared with that in saline group;BALF eotaxin level declined too. Serum IL - 5 and total IgE level were also significantly reduced; IL- 5 mRNA expression in lung significantly decreased. Eotaxin and its mRNA expression in lung were not decreased significantly. Conclusion MK exerts its anti - inflammatory effect mainly through the suppression of IL- 5 and IgE production.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第21期1452-1454,共3页
Journal of Applied Clinical Pediatrics
基金
广东省自然科学基金项目资助(5001776)
