摘要
目的:应用随机扩增多态DNA聚合酶链反应(RAPD-PCR)技术分析自发性高血压大鼠(SHR)的基因组多态性。方法:运用100条引物对WKY和SHR的DNA进行RAPD-PCR扩增,琼脂糖凝胶电泳观察。结果:与WKY相比,100条引物中有13条引物出现基因组多态性,SHR扩增产物的电泳条带出现增加、丢失、移位和密度改变,其它87条引物扩增结果无明显改变。结论:SHR存在基因组多态性,RAPD-PCR方法可用于检测SHR的基因组多态性。
AIM： To assess the genomic polymorphism of spontaneously hypertensive rats （SHR） by using random amplified polymorphic DNA PCR （RAPD- PCR）. METHODS： The DNA of WKY rats and SHR was amplified with RAPD - PCR using 100 single primers. The PCR products were resolved with electrophoresis on agarose gel and stained with ethidium bromide. RESULTS： Thirteen of the 100 single primers resulted in genomic polymorphism. In the RAPD method, genetic alterations appeared as either loss or gain of a band, shift of a band, or decrease or increase in intensities of a band of SHR DNA relative to WKY rat tissue DNA. The other primers produced amplification products were not different in two groups. CONCLUSION： It is suggested that SHR present genomic polymorphism. RAPD - PCR is an effective technique to detect genomic polymorphism in SHR.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第11期2156-2158,共3页
Chinese Journal of Pathophysiology
关键词
基因组多态性
大鼠
自发性高血压
Genomie polymorphism
Rats
Spontaneously hypertension
