摘要
目的：探讨内皮素（ＥＴ）与肾小球肾炎的关系，方法：建立改良慢性血清病大鼠系膜增生性肾炎动物模型，采用￣１２５Ｉ－ＥＴ放射免疫分析法测定肾炎组与付照组右心血和肾静脉血中ＦＴ－１的水平。应用免疫组化及免疫电镜方法进行ＦＴ－１的定位研究，结果：肾炎组肾静脉血及右心血ＦＴ－１血浆水平均高于对照组（Ｐ＜０．０５），而且肾静脉血ＦＴ－１含量明显高于右心血（Ｐ＜０．００１）。ＦＴ－１广泛存在于肾脏的各种固有成分中。结论：肾炎时肾阻自身合成和分泌的ＦＴ是血浆ＦＴ的主要来源，炎性增生的肾小球固有细胞和肾小管上皮细胞分泌的ＦＴ是肾脏局部ＦＴ的主要来源。
Objective:To explore the ralationship between endothelin (ET)and glomerulonephritis.Methods:Rat model of mesangioproliferative glomerulonephritis(MsPGN)was established.The ET-1 levels in the right ventricle plasma were measured and compared with those in renal vein by125I-radioim-munoassay(RIA).The distribution of FT-1 in kidney was detected by immunohistochemical staining and immunoelectron-microscopy.Results:The plasma level of FT-1 was significantly greater in experi-mental group than that of control group both in the ventricle and renal vein.Level of ET-1 in renal vein was significantly higher than that in the right ventricle,Immunohistochemistry staining and immuno-electron-microscopy showed that ET-1 widely disposed many components of kidney. Conclusion:The plasma of ET-1 derivey mainly from proliferative mesangial cells and renal tubular secretion in MsPGN.
出处
《北京医科大学学报》
CSCD
1996年第5期334-336,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
肾
内皮素
定位
肾小球肾炎
Kidney/Chem:Fndothelins/anal:Fndothelins/blood
