摘要
以40mmoL/L硼砂溶液为缓冲液,利用高效毛细管电泳(HPCE)法,建立不同产地金银花的HPCE指纹图谱并测定其中绿原酸的含量。工作电压为17kV,温度为25℃,压力进样时间为6s。不同产地金银花药材中绿原酸的含量为3.2%4.4%。通过对HPCE指纹图谱共有峰数据进行判别分析和聚类分析,可以将不同产地的金银花进行判别分类。HPCE指纹图谱可以用于较全面地考察金银花药材的质量。
Finger prints of Flos Lonicerae Japonicae were established and the content of chlorogenic acid were determined by high performance capillary electrophoresis （HPCE）. The analysis was performed in 40 mmoL/L borax solution, running voltage was 17 kV, column temperature was 25℃ and the sample was injected by pressure for 6 s. With the contents of chlorogenic acid varied from 3.2% -4.4%, the Flos Lonicerae Japonicae from different resources was able to be classified according to the HPCE finger print data processed by stepwise discriminant analysis and clustering analysis. The HPCE finger print was suitable for Flos Lonicerae Japonicae quality control.
出处
《化学分析计量》
CAS
2006年第6期66-68,共3页
Chemical Analysis And Meterage
关键词
金银花
绿原酸
高效毛细管电泳
指纹图谱
Flos Lonicerae Japonicae, chlorogenic acid, HPCE, finger print
