摘要
目的研究鹅膏毒肽对大鼠多组织细胞DNA的损伤及二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)的拮抗效应。方法将30只健康成年SD大鼠随机分成3组,对照组、鹅膏毒肽染毒组、鹅膏毒肽染毒+EPA+DHA组。雌雄各半。采用腹腔注射染毒,应用单细胞凝胶电泳检测各组大鼠的外周血淋巴细胞、肝细胞、肺细胞和脑细胞DNA的损伤。结果鹅膏毒肽染毒组大鼠外周血淋巴细胞、肝细胞、肺细胞和脑细胞的彗星细胞尾长、受损DNA所占百分比(尾DNA%)均高于对照组(P<0.05),而鹅膏毒肽染毒+EPA+DHA组大鼠外周血淋巴细胞、肝细胞、肺细胞及脑细胞的彗星细胞尾长、尾DNA%与对照组相比变化不明显。结论鹅膏毒肽可致大鼠多组织细胞DNA损伤,EPA和DHA可拮抗这种损伤。
Objective To study the adverse effect of amatoxins on DNA in the histioeytes of rats and the antagonism of eicosapentaenoic acid （EPA）, docosahexaenoie acid （DHA）. Methods SD rats were randomly divided into three groups, the number of males and females was the same. The rats in the experimental groups were respectively treated with amatoxins（0.1 mg/kg bw, i.p.） and amatoxins added EPA, DHA （both were 10 mg/kg bw, i.p.） for 10 days. Single cell gel-electrophoresis was employed to test the damage of DNA in the histiocytes. Results In the group treated with amatoxins, DNA damage in the peripheral lymphocyte, hepatocytes, lung cells ,brain cells was more seriously compared with the control, but no significant DNA damage was seen in the group treated with amatoxins added EPA, DHA. Conclusion Amatoxins may induce DNA damage and eieosapentaenoic acid, docosahexaenoie acid have an antagonism to the adverse effect of amatoxins.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第6期537-538,共2页
Journal of Environment and Health
基金
湖南省卫生厅科研基金资助项目(B2005120)
湖南省教育厅科研基金资助项目(05D030)
湖南环境生物职业技术学院院长科研基金资助项目(Z05-11)
