摘要
目的研究用多重PCR的方法鉴定产气荚膜梭菌及分型毒素,为开展基因诊断做好准备。方法根据GenBank已经发表的产气荚膜梭菌毒素基因序列,设计出针对CPα、CPβ、CPE毒素基因的3对特异引物,运用多重PCR的方法鉴定出产气荚膜梭菌并对其毒素基因进行分型。结果经电泳鉴定,多重PCR成功的扩增出预先设计的3条特异的目的条带。结论所设计的多重PCR反应体系能够得到产气荚膜梭菌的特异序列,同时能够鉴定分型3种对人致病的毒素序列,为进一步开展基因诊断打下基础。
OBJECTIVE To search a method for identifying Clostridiurn perfringens and genotyping their toxin for gene diagnosis by multiplex PCR. METHODS The mutiplex PCR was developed with three sets of primers designed based on the sequences of three C. perfringens toxin genes（ CPα, CPβ and CPE ） published in GenBank to identify C. perfringens and genotype their three toxin genes. RESULTS Three expected sequences were obtained successfully by multiplex PCR and identified by electrophoresis. CONCLUSIONS The specific sequences of C. perfringens could be amplified and their three genes of toxins could be identified by this multiplex PCR system. Such method should be helpful for developing gene diagnosis well.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2006年第11期1310-1312,共3页
Chinese Journal of Nosocomiology
基金
国家863计划重大专项(2002AARZ2023)
国家自然科学基金面上项目(30400107
30270388)
军队"十五"课题(01MA180
01LO65)
重庆市攻关课题(2004-20-12)
