摘要
从海南省各市县香蕉种植区采集发病的香蕉和粉蕉植株近地面茎段维管束病变的样本,经分离纯化获14个单孢菌株。经寄主致病性测定表明:粉蕉分离物皆为香蕉枯萎病菌1号生理小种,香蕉分离物则为4号生理小种。利用氯酸钾诱变这14个枯萎菌株共产生104个抗氯酸盐突变体,诱发获得67个nit突变株,其中nitl型58株,nitM型6株,nit3/nit8型3株。将获得的nit突变株做营养亲和性配对反应,可将14个菌株分为2个亲和群,其中VCG1包括7个菌株,且全为1号生理小种,VCG2则由7个4号小种菌株组成。
Fourteen single-spore isolates of Fusarium oxysporum f. sp. cubense （Foc） were collected from main banana producing region in Hainan. Inoculation of identified host assay indicated that isolates from Fenjiao belong to the typical race 1, and isolates from Xiangjiao were race 4. Vegetative compatibility of banana Fusarium wilt was determined by the technology of nitrate reductase deficiency. All of 14 isolates produced 104 chlorate-resistant sectors on KPS media and 67 nitrate nonutilizing mutants （nit mutant）, which were recovered by transferring the chlorate-resistant sectors to the MM media containing nitrate as single nitrogen source. Among 67 nit mutants, 58 nit mutants were nit 1, 6 nit mutants were nit M, and 3 nitmutants were nit 3/nit 8. Complement of vegetative compatibility tests were carried out on MM media by pairing the nit mutants from the same strain and from different strains. 14 strains were assigned to 2 vegetative compatibility groups （VCGs）, 7 strains （ race 1） in VCG 1,7 strains （race 4） in VCG 2.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第6期830-833,共4页
Journal of Fruit Science
基金
中国热带农业科学院科技基金资助项目(Rky0612)
海南省重点科技项目(05303)。
关键词
香蕉枯萎病菌
nit突变株
营养亲和群
Fusarium oxysporum f. sp. cubense
nit mutants
Vegetative compatibility groups
