摘要
以外消旋扁桃酸为底物,筛选出一株短杆菌Brevibacteriumsp.CCSYU10011,该菌能转化外消旋扁桃酸为(R)-扁桃酸.用全细胞转化法研究发现,其转化过程是不对称降解过程,即选择性降解了(S)-扁桃酸,进而获得(R)-扁桃酸.考察了温度、pH、底物浓度及细胞量等因素对(S)-扁桃酸降解的影响,转化结束后,收率为48.7%,对映体过量值(e.e.)可达99%.
（R）-mandelic acid is a useful chiral synthon for the production of various pharmaceutical. In order to prepare （R）-mandelic acid from rac-mandelic acid, Brevibacterium sp. CCSYU10011 was screened from various microorganisms, of which the resting cells could convert the recreate to （R）-isomer. The process of bioconversion showed that the microorganism asymmetricly degraded （S）-mandelic acid from the racemate and （R）-mandelic acid was maintained. The factors affecting biotransformation efficiency were investigated, including temperature, pH, substrate concentration and cell biomass. Under the optimal conditions, 48.7% yield and 99% enantiomeric excess value were obtained.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2006年第5期818-821,共4页
The Chinese Journal of Process Engineering
基金
国家重点基础研究发展规划(973)基金资助项目(编号:2003CB716008)
新世纪优秀人才支持计划资助项目
长江学者和创新团队发展计划资助项目(编号:IRT0532)
