摘要
背景与目的:某些小剂量中药和小剂量化疗药物可选择性地抑制血管内皮细胞的功能。因此本文探讨参麦注射液联合应用羟基喜树碱对体内外血管生成的抑制作用。方法:以人肝癌细胞HepGⅡ作为对照,采用MTT法和三维血管模型观察参麦注射液联合羟基喜树碱对体外人微血管内皮细胞(HMVEC)增殖和迁移的影响;利用鸡胚尿囊膜(CAM)模型观察参麦注射液联合羟基喜树碱对体内鸡胚绒毛尿囊膜血管生成的影响。结果:40μl/ml参麦注射液和20ng/ml羟基喜树碱对血管内皮细胞增殖的抑制率分别为18%、31.7%,联合应用后的抑制率为43.6%;40μl/ml参麦注射液和20ng/ml羟基喜树碱对HepGⅡ细胞的抑制率分别为1.2%、0.9%,联合应用后的抑制率为19.4%;40μl/ml参麦注射液和20ng/ml羟基喜树碱对血管内皮细胞有抑制出芽的作用,联合应用具有显著的协同效应,且与作用时间呈正相关(r=0.988,P=0.007);40μl/ml参麦注射液和20ng/ml羟基喜树碱对鸡胚尿囊膜新生血管抑制率分别为30%、40%,联合应用后的抑制率为60%。结论:①小剂量参麦注射液(40μl/ml)、羟基喜树碱(20ng/ml)在体内外具有抑制血管生成作用,而对肝癌细胞无明显抑制作用,显示出明显差异性细胞毒作用;②小剂量参麦注射液(40μl/ml)联合羟基喜树碱(20ng/ml)在体外具有抑制HMVEC增殖和迁移协同作用,体内具有抑制血管生成协同作用。
Background and purpose： It has been reported that some low dose traditional Chinese medicine along with low dose chemotherapy drugs could selectively inhibit the function of vascular endothelial cell. This experiment aims to study the impact of Shenmai combined with hydroxycamptothecin on angiogenesis in vitro and vivo. Methods： HepG Ⅱ cells was used as control group, proliferations of human microvascular endothelial cell（HMVEC） were measured by MTY colorimetric assay. After treated by Shenmai combined with hydroxycamptothecine. Migration of HMVEC was observed by a microcarrier-based three-dimensional angiogenesis model in vitro. We also studied the impact of the combination on anglogenesis in chick embryo choriallantoic membrane （CAM） model. Results： Shenmai injection （40μl/ml） and hydroxycamptothecine （20ng/ml） can inhibit the prolifetation of HMVEC; hydroxycamptothecine alone and 43.6% for the combination, the rate of inhibition were 18% for Shenmai alone ,31.7% for respectively, they could also inhibit the prolifetation of HepG Ⅱ cell; the rate of inhibition were 1.2% ,0.9% and 19.4%, respectively; there were synergistic effect exist if Shenmai injection （40μl/ml） used with hydroxycamptothecine （20ng/ml） in HMVEC; the above concentration of Shenmai and hydroxycamptothecine could also inhibit the sprouts of HMVEC, they had synergistically interacted with each other and the effect was correlated with incubation time （ the coefficient was 0.988, P = 0. 007） ; the same response could be observed in CAM model in terms of angiogenesis, the rate of inhibition were 30% for Shenmai alone ,40% for hydroxycamptothecine alone and 60% for the combination, respectively. Conclusions： ①Low dose Shenmai injection （40μl/ml） ,hydroxycamptothecine （20ng/ml） can inhibit angiogenesis both in vitro and vivo, but had no effects to HepG Ⅱ cell, it seems to be cell lineage dependent; ②Low dose Shenmai injection （40μl/ml） combined with hydroxycamptothecine （20ng/ml） can synergistically inhabit the migration of HMVEC in vitro and angiogenesis in vivo.
出处
《中国癌症杂志》
CAS
CSCD
2006年第11期953-957,共5页
China Oncology
基金
南京市科技局国际合作项目(200301112).
关键词
参麦注射液
羟基喜树碱
内皮细胞
抗血管生成
shenmai injection
hydroxycamptothecin
endothelial cell
antiangiogenesis
