摘要
目的观察己酮可可碱(PTX)和阿苯达唑(ABZ)单独及联合用药对小鼠继发性泡球蚴病的疗效。方法对小鼠继发性泡球蚴病进行药物治疗,各治疗组药物用量分别为:ABZ组50mg/(kg·d);PTX高剂量组360mg/(kg·d);PTX低剂量组180mg/(kg·d);联合组ABZ50mg/(kg·d)+PTX180mg/(kg·d);感染对照组(未治疗组)和空白对照组均给予等体积生理盐水,用小鼠灌胃针经口每天灌胃给药1次,连续治疗100d后(其间14只死亡),检测各小鼠泡球蚴湿重、抑囊率及小鼠血清细胞因子转化生长因子-β(TGF-β)、白细胞介素-2(IL-2)和IL-10;并对泡球蚴组织进行病理组织学和超微结构观察。结果PTX在体外能有效地杀灭原头节(高剂量组为100%),在体内对泡球蚴抑制作用虽较弱(高剂量组为37%),但能增强小鼠的免疫力。联合用药对泡球蚴有明显的抑制作用,抑囊率为88%,ABZ抑囊率为58%(P<0.05)。结论PTX联合ABZ治疗小鼠继发性泡球蚴病疗效明显优于ABZ。
Objective To observe the effect of pentoxifylline （PTX）, albendazole （ABZ）, and a combination of PTX and ABZ in mice infected with Echinococcus multilocularis （E.M）. Methods The first part of the experiment was to observe the in vitro effect of PTX on the cultured E.M protoscolex. In the second part, mice were infected by abdominal inoculation of E.M and divided into groups given by ABZ 50 mg/kg·d, PTX 360 mg/kg·d, PTX 180mg/kg·d, and a combined regimen ABZ 50 mg/（kg·d）＋PTX 180 mg/（kg·d）, Another infected group and a uninfected group served as controls which received normal saline only. 100 days post-treatment, the mice were sacrificed for further observation. Indicators included wet weight of the cyst, cyst inhibition rate, level of serum cytokines TGF-β determined by ELISA, IL-2 and IL-10 determined by radio-immunoassay （RIA）. Results The inhibition rate on cysts of the combined ABZ and PTX was 88%, considerably higher than 58 % of the group ABZ. The serum TGF-β and IL-10 decreased and IL-2 increased after treatment in comparison to the controls. Conclusion The PTX and ABZ combination shows better effect on E.multilocularis infection than that of single ABZ. PTX might help increase immunity of the mice.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第5期333-336,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
泡球蚴病
己酮可可碱
阿苯达唑
细胞因子
Echinococcus multilocularis
Pentoxifylline
Albendazole
Cytokines
